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Protein translocation across biological membranes
Energy requirements of protein translocation
Electrical currents induced by ion pumps on black lipid membranes
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absorption AcChR Acta activity addition amino acid ATP-hydrolysis ATPase bacteriorhodopsin bilayer Biochem Biochemistry Biol Chem biological membranes Biophys Ca2+ Ca2+-ATPase catalytic Cell channel charge chloroplasts complex concentration conformational change constant curve cytochrome c oxidase cytosol detergent dialysis dimer electric field EMBO energy enzyme equation equilibrium eukaryotic FEBS Lett fluorescence function helices heme high affinity histidine hydrophobic hysteresis inner membrane interaction Kadenbach kinetics ligand lipid measured mechanism membrane potential membrane proteins method mitochondrial molecular molecules monomer monomeric Natl Acad Nature London Neupert observed obtained oligomeric oriented oxidized photocycle plasma membrane polypeptide potential difference dependence precursor proteins presence probe Proc proton pumping purple membrane R.A. Capaldi rate of ATP-synthesis reaction receptor reconstituted redox residues S.I. Chan shown signal sequence spectroscopic structure substrate subunit surface potential thylakoid translocation transmembrane transport vesicles