Distance Measurements in Biological Systems by EPR

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Lawrence Berliner, Sandra S. Eaton, Gareth R. Eaton
Springer Science & Business Media, Mar 31, 2001 - Medical - 614 pages
Distance measurements in biological systems by EPR The foundation for understanding function and dynamics of biological systems is knowledge of their structure. Many experimental methodologies are used for determination of structure, each with special utility. Volumes in this series on Biological Magnetic Resonance emphasize the methods that involve magnetic resonance. This volume seeks to provide a critical evaluation of EPR methods for determining the distances between two unpaired electrons. The editors invited the authors to make this a very practical book, with specific numerical examples of how experimental data is worked up to produce a distance estimate, and realistic assessments of uncertainties and of the range of applicability, along with examples of the power of the technique to answer biological problems. The first chapter is an overview, by two of the editors, of EPR methods to determine distances, with a focus on the range of applicability. The next chapter, also by the Batons, reviews what is known about electron spin relaxation times that are needed in estimating distances between spins or in selecting appropriate temperatures for particular experiments. Albert Beth and Eric Hustedt describe the information about spin-spin interaction that one can obtain by simulating CW EPR line shapes of nitroxyl radicals. The information in fluid solution CW EPR spectra of dual-spin labeled proteins is illustrated by Hassane Mchaourab and Eduardo Perozo.
 

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Contents

Introduction
4
Effect ofRapidlyRelaxing Metal on Spin Echo Dephasing Tm
Dipolar Interactions
Distributions of Distances
Processes that Contribute to T1 in Immobilized Samples
Processes that Contribute to Tm in Immobilized Samples
Processes that Contribute to T1 and T2 in Fluid Solution
Chapter 10
Instrumentation
Examples
Discussion
Appendices
2+1 Pulse Sequence as Applied for Distance and Spatial Dlstrlbutl
Chapter 6
Theory

ExperimentalData
Summary
Chapter 3
5
6
Chapter 4
Electron Spin Echo Envelope Modulation of a Photoinduced Radi
Chapter 5
Structural Modeling Using EPRDetermined Distances
Investigating Membrane Protein Dynamics using Dipolar
Chapter 9
DoubleQuantum ESR and Distance Measurements
Conclusion
Chapter ll
u1ul1
DEER on Nitroxides
Chapter 2
Distance Measurements in Photosynthetic Reaction Centers
Introduction
Structural Investigations in Photosynthetic Reaction Centers
Conclusion
Index
Copyright

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About the author (2001)

Dr. Lawrence J. Berliner is currently Professor and Chair of the Department of Chemistry and Biochemistry at the University of Denver after retiring from Ohio State University, where he spent a 32-year career in the area of biological magnetic resonance (EPR and NMR). He is the Series Editor for Biological Magnetic Resonance, which he launched in 1979.