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Construction of Pichia pastoris Alcohol OxidaseDefective Mutants by Gene
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2-plm circle Acad acetate ADH2 agar alluvius amino acid analysis AOX1 assay auxotrophic Bacillus subtilis BamHI Biochem Biol brewing strain brewing yeast brewing yeast strains CAR1 carbon source cellulose chromosomal cleavage cloned Cold Spring Harbor construction culture deletion derepressed detected diastaticus diploid DNA fragments DNA polymerase DNA replication DNA sequence dsDNA EcoRI encoded enzyme Escherichia coli eukaryotic fermentation FLP recombination fractions function fusion gene expression genetic genome glucoamylase glucose glycosylation haploid heterologous HindIII homology host hybrid incubation industrial yeast insertion invertase isolated KEX2 lane malate malic acid medium metabolism methanol molecules mutant Natl o-factor pastoris peptide phenotype Pichia plasmids containing polyploid potassium acetate prepro primase Proc promoter protein region REP1 REP2 REP3 repression resistance resolvase activity Saccharomyces cerevisiae Schizosaccharomyces pombe spheroplasts sporulation ssDNA cellulose column stability structure substrate SUC2 Table tetrad transcript Ty element vector vitro wild-type yeast transformants