Biophysical Labeling Methods in Molecular Biology
This monograph describes the theoretical bases and experimental prerequisites for methods such as spin fluorescence, triplet, Mössbauer, photochromic and electron-density labeling, including the procedures used to obtain specifically modified proteins, enzymes, biomembranes, nucleic acids, and other biological molecules. The fundamentals of the physical theory behind each technique is explained and details are given of how to interpret the experimental data obtained. Special sections deal with critical reviews of recent data on the structure, molecular dynamics and conformational transitions of biological molecules. Each section concludes with a discussion of the results obtained from these techniques in connection with various problems of enzyme catalysis, electron transfer, molecular biophysics and molecular biology. The uses that labeling techniques can be put to for the investigation of whole cells and tissues are also discussed.
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1 The method of spin labeling
2 Doublelabeling techniques
3 Fluorescent labeling methods
4 Triplet labeling methods
5 Mossbauer spectroscopy electron scattering and other labeling methods
structure dynamics and mechanism of action
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absorption acceptor active center anisotropic Antsiferova atoms bilayer Biochemistry biological membranes biological objects biophysical chemical chromophores coefﬁcient collisions complex components compounds conformational correlation cytochrome P-450 determined dioxygen dipole dipole—dipole distance Dobretsov 1989 donor dynamic effects efﬁciency electron transfer electron-scattering labels energy transfer enzyme equation erythrocyte ESR signal ESR spectrum exchange interaction exciplexes experimental Figure ﬁrst ﬂuorescence ﬂuorescent probes frequency g-factor heme hydrophobic hyperﬁne intensity ions kinetics Kotelnikov Kulikov labeling method labels and probes Likhtenshtein line width lipid liposomes luminescent lysozyme macromolecule magnetic ﬁeld membranes mobility modiﬁed molecular molecular dynamics molecules Mossbauer myoglobin nitrogenase nitroxide fragment nitroxide radicals nuclear nucleic acids orientation paramagnetic parameters phosphorescence physical labels polarity portions processes protein protein globule protons quenching rate constant reﬂected relaxation shift resonance rhodopsin rotational diffusion Russian SH group signiﬁcant solution speciﬁc spectra spectroscopy spin labels spin probes spin—spin structure substrate techniques temperature dependence transition