Biophysical Tools for Biologists: In Vivo Techniques
Academic Press, Jan 19, 2009 - Science - 704 pages
Driven in part by the development of genomics, proteomics, and bioinformatics as new disciplines, there has been a tremendous resurgence of interest in physical methods to investigate macromolecular structure and function in the context of living cells. This volume in Methods in Cell Biology is devoted to biophysical techniques in vivo and their applications to cellular biology. The volume covers methods-oriented chapters on fundamental as well as cutting-edge techniques in molecular and cellular biophysics. This book is directed toward the broad audience of cell biologists, biophysicists, pharmacologists, and molecular biologists who employ classical and modern biophysical technologies or wish to expand their expertise to include such approaches. It will also interest the biomedical and biotechnology communities for biophysical characterization of drug formulations prior to FDA approval.
* Describes techniques in the context of important biological problems
* Delineates critical steps and potential pitfalls for each method
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Biophysical Tools for Biologists: In vivo techniques
John Joseph Correia,H. William Detrich
No preview available - 2008
Acad acceptor actin activation adhesion algorithm analysis Axelrod beads beam binding biochemical biological Biophys cantilever Cell Biol Cell Biology cellular Chem concentration confocal microscopy coverslip cross-linked cytoplasm cytoskeleton detection distribution diVerent diVerential diVusion diYcult donor dynamics elastic electron tomography emission energy evanescent field eVect excitation eYciency F-actin filaments FLIM fluorescence correlation spectroscopy fluorescence microscopy fluorescent protein fluorophore focal force FPALM FRET function green fluorescent protein illumination imaging importin intensity interactions islet kinetics laser living cells measurements mechanical method microtubules molecular molecules motor nanoparticles Natl networks neurons optical optical tweezers photobleaching photon pixel plasma membrane polarized probe Proc quantitative Raman receptors reconstruction region resolution sample scanning sections sensor signal simulation single single-molecule spatial specimen spectra stochastic structure surface suYcient techniques tether tetra-Cys CRABP tilt tion TIRF tissue total internal reflection two-photon vitro vivo volume wavelength