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Strategies in Downstream Processing
Protein Stability in Downstream Processing
Production of Transgenic Protein
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activity amino acid analysis antibody assays bacteria batch bead mill Biochem Biol Chem biological biological safety cabinets bioprocessing Biotechnol Biotechnology buffer C-terminal carbohydrate carbohydrate chains carriers cell culture cell disruption cell lines centrifuge chemical chromatography coli column concentration containment cytokines deamidation debris degradation denaturation disulfide bonds downstream processing Edman degradation effect enzymatic enzymes equipment factors fermentation filters filtration fluidized bed fraction glycans glycoprotein glycosylation homogenizer HPLC human hydrophobic IFN-a inclusion bodies increase ions MALDI mass membrane methods microcarriers molecular molecules monosaccharide N-linked oligosaccharides operations oxygen packed bed parameters peptide polypeptide proteases protein purification reaction reactor recombinant recovery redox potential reduced refolding release removal residues sample scale-up seal separation sequence soluble solution solvent specific stability standard step sterilization structure surface Table techniques tein temperature therapeutics tion validation valve yeast yield