Bioseparations of Proteins: Unfolding/Folding and Validations

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Elsevier, Nov 27, 1997 - Science - 346 pages
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This book covers the fundamentals of protein inactivation during bioseparation and the effect on protein processing. Bioseparation of Proteins is unique because it provides a background of the bioseparation processes, and it is the first book available to emphasize the influence of the different bioseparation processes on protein inactivation.
Bioseparation of Proteins covers the extent, mechanisms of, and control of protein inactivation during these processes along with the subsequent and essential validation of these processes. The book focuses on the avoidance of protein (biologicalproduct) inactivation at each step in a bioprocess. It compares protein inactivation exhibited during the different bioseparation processes by different workers and provides a valuable framework for workers in different areas interested in bioseparations.
Topics include separation and detection methods; estimates of protein inactivation and an analysis of this problem for different separation processes; strategies for avoiding inactivation; the molecular basis of surface activity and protein adsorption,process monitoring, and product validation techniques; and the economics of various bioseparation processes and quality control procedures.

Key Features
* Protein inactivation and other aspects of biological stability are critical to an effective bioseparation process; This book is a detailed and critical review of the available literature in an area that is essential to the effectiveness, validation, and economics of bioseparation processes for drugs and other biological products; Conveniently assembled under one cover, the survey of the literature and resulting perspective will greatly assist engineers and chemists in designingand improving their own processes; Key features of the text include:
* detailed data on biological stability under various bioseparation conditions
* extensive case studies from the literature on separation processes, validation, and economics
* simplified analysis of protein refolding and inactivation mechanisms
* consideration of adsorption theories and the effect of heterogeneity
* coverage of both classical and novel bioseparation techniques, including chromatographic procedures
 

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Contents

2 Steps in Bioseparation Processes
19
3 HighResolution Fractionation Processes
61
4 Interfacial Protein Adsorption and Inactivation during Bioseparation
101
5 Protein Inactivations during Chromatographic Methods of Separation
135
6 Protein Inactivations during Novel Bioseparation Techniques
177
7 Adsorption Influence on Bioseparation and Inactivation
213
8 Applications and Economics of Bioseparation
259
9 Protein Refolding and Inactivation during Bioseparation
287
10 Validation of the Production of Biological Products
313
INDEX
339
Copyright

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Page 16 - Volume 3 STABILITY OF PROTEIN PHARMACEUTICALS, Part B: In Vivo Pathways of Degradation and Strategies for Protein Stabilization Edited by Tim J.
Page 15 - Parenteral Delivery of Peptide and Protein Drugs, In Peptide and Protein Drug Delivery, Lee, VHL, Ed., Marcel Dekker: New York, pp.
Page 15 - STABILITY OF PROTEIN PHARMACEUTICALS, Part A: Chemical and Physical Pathways of Protein Degradation Edited by Tim J.

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About the author (1997)

Dr. Satinder Ahuja is a leading expert on improving water quality. He earned his PhD in analytical chemistry from the University of the Sciences, Philadelphia. He worked for Novartis Corp. in various leadership positions for over 25 years while simultaneously teaching and guiding research as an adjunct professor at Pace University for over 10 years. As president of Ahuja Consulting, he advises on quality issues relating to chemicals/pharmaceuticals and water. Dr. Ahuja has published numerous papers and more than 20 books. His latest books are “Chemistry and Water: The Science Behind Sustaining the World’s Most Crucial Resource (Elsevier, 2016), “Food, Energy, and Water: The Chemistry Connection" (Elsevier, 2015), and “Water Reclamation and Sustainability" (Elsevier, 2014). Others include “Comprehensive Water Quality and Purification" (Elsevier, 2013), "Monitoring Water Quality: Pollution Assessment, Analysis, and Remediation" (Elsevier, 2013), "Novel Solutions to Water Pollution" (ACS Symposium Volume, 2013), "Handbook of Water Purity and Quality" (Elsevier, 2009), and "Arsenic Contamination of Water: Mechanism, Analysis, and Remediation" (Wiley, 2008). A member of the executive committee of the Rivers of the World Foundation (ROW), Dr. Ahuja has organized numerous global symposia on improving water quality, including presentations for the American Chemical Society and UNESCO.

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