Bioseparations of Proteins: Unfolding/Folding and Validations
This book covers the fundamentals of protein inactivation during bioseparation and the effect on protein processing. Bioseparation of Proteins is unique because it provides a background of the bioseparation processes, and it is the first book available to emphasize the influence of the different bioseparation processes on protein inactivation.
Bioseparation of Proteins covers the extent, mechanisms of, and control of protein inactivation during these processes along with the subsequent and essential validation of these processes. The book focuses on the avoidance of protein (biologicalproduct) inactivation at each step in a bioprocess. It compares protein inactivation exhibited during the different bioseparation processes by different workers and provides a valuable framework for workers in different areas interested in bioseparations.
Topics include separation and detection methods; estimates of protein inactivation and an analysis of this problem for different separation processes; strategies for avoiding inactivation; the molecular basis of surface activity and protein adsorption,process monitoring, and product validation techniques; and the economics of various bioseparation processes and quality control procedures.
* Protein inactivation and other aspects of biological stability are critical to an effective bioseparation process; This book is a detailed and critical review of the available literature in an area that is essential to the effectiveness, validation, and economics of bioseparation processes for drugs and other biological products; Conveniently assembled under one cover, the survey of the literature and resulting perspective will greatly assist engineers and chemists in designingand improving their own processes; Key features of the text include:
* detailed data on biological stability under various bioseparation conditions
* extensive case studies from the literature on separation processes, validation, and economics
* simplified analysis of protein refolding and inactivation mechanisms
* consideration of adsorption theories and the effect of heterogeneity
* coverage of both classical and novel bioseparation techniques, including chromatographic procedures
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3 HighResolution Fractionation Processes
4 Interfacial Protein Adsorption and Inactivation during Bioseparation
5 Protein Inactivations during Chromatographic Methods of Separation
6 Protein Inactivations during Novel Bioseparation Techniques
7 Adsorption Influence on Bioseparation and Inactivation
acids adsorbed adsorbed protein affinity affinity chromatography aggregation analysis antibodies authors emphasized authors indicated authors noted binding Biochem Bioeng biological products bioproducts bioseparation bioseparation process bioseparation protocol Bioseparation step Biotechnol Biotechnology Briefly analyze Briefly describe buffer cell disruption chaperonins Chem Chemical chro Chromatogr chromatography Colloid Interface Sci column concentration conformational changes cost denaturation downstream processing drug effects elution enzyme Enzyme Microb example extraction fermentation fibrinogen folding gel filtration glucose isomerase heterogeneity HPLC hydroxylapatite inactivation increase initial ion-exchange isoelectric point isotherm kinetics large-scale ligand lipase lysozyme macromolecules membrane method minimize molecular monoclonal antibodies obtained parameters partitioning peptides polymer precipitation procedures products of interest protein adsorption protein molecules Protein Purification purification factor Purification Protocol recombinant recovery refolding reverse micellar reverse micelles Sadana scale-up SDS-PAGE separation significant solubilization solution solvent specific activity stability structure studies surface surfactant technique Technol teins tion ultrafiltration utilized validation yield
Page 16 - Volume 3 STABILITY OF PROTEIN PHARMACEUTICALS, Part B: In Vivo Pathways of Degradation and Strategies for Protein Stabilization Edited by Tim J.