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INCORPORATION OF MEVALONATE AND ACETATE INTO STEROLS
TESTICULAR TISSUE FROM RATS
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0.1 M Tris-HCl acetone acetone powder added addition alcohol alkaline phosphatase alumina assayed Biochem Biol C-prenol C-prenol-PP carbon dioxide catalyze centrifuged Cgy sterols Chem cholesterol cholesterol and companions chromatographed column concentration cpm/mg crude enzyme dehydrogenase demethylation demethylation of lanosterol digitonin eluted enzyme esters extract farnesoic acid farnesol farnesyl pyrophosphate farnesyl-PP final volume flask contained formation glucose glutathione hour at 37 hydrolysis incorporation of acetate incubated inhibition inhibitors inorganic pyrophosphatase IPPP isopentenyl-PP labeled acetate labeled carbon dioxide labeled lanosterol lanosterol and companions Maximal methanol mevalonate-5-P mevalonic acid mg protein microsomes mitochondria mumoles nerolidol nonsaponifiable lipids obtained oxidation partially purified pentane phosphate buffer Popjak prenol pyrophosphate phosphatase prenol-PP phosphatase protein purified enzyme radioactivity rat liver rat testicular tissue rate of demethylation recrystallized Sephadex solution squalene sterols and steroids Stoichiometry substrate supernatant fraction suspension synthesis Table testicular microsomes testicular sterols testis testosterone TPNH Triparanol unlabeled yeast yield