Biotechnology: A Laboratory Course

Front Cover
Academic Press, Dec 2, 2012 - Science - 250 pages
0 Reviews
Biotechnology: A Laboratory Course is a series of laboratory exercises demonstrating the in-depth experience and understanding of selected methods, techniques, and instrumentation used in biotechnology. This manual is an outgrowth of an introductory laboratory course for senior undergraduate and first year graduate students in the biological sciences at The University of Tennessee.

This book is composed of 19 chapters and begins with some introductory notes on record keeping and safety rules. The first exercises include pH measurement, the use of micropipettors and spectrophotometers, the concept of aseptic technique, and preparation of culture media. The subsequent exercises involve the application of the growth curve, the isolation, purification, and concentration of plasmid DNA from Escherichia coli, and the process of agarose gel electrophoresis. Other exercises include the preparation, purification, and hybridization of probe, the transformation of Saccharomyces cerevisiae, the transformation of E. coli by plasmid DNA, and the principles and applications of protein assays. The final exercises explore the ?-galactosidase assay and the purification and determination of ?-galactosidase in permeabilized yeast cells.

This book is of great value to undergraduate biotechnology and molecular biology students.
 

What people are saying - Write a review

We haven't found any reviews in the usual places.

Contents

Chapter 19 βGalactosidase Purification
135
Appendix 1 Alternative Protocolsand Experiments
151
Appendix 2 Buffer Solutions
167
Appendix 3 Preparation of Buffers and Solutions
170
Appendix 4 Properties of Some Common Concentrated Acids and Bases
174
Appendix 5 Use of Micropipettors
175
Appendix 6 Safe Handling of Microorganisms
178
Appendix 7 List of Cultures
181

The Streak Plate
27
Chapter 5 Preparation of Culture Media
33
Chapter 6 The Growth Curve
41
The MiniPrep
47
Chapter 8 Purification Concentration and Quantitation of DNA
53
The MaxiPrep
61
Chapter 10 Restriction Digestion and Agarose Gel Electrophoresis
71
Chapter 11 Southern Transfer
81
Chapter 12 Preparation Purification and Hybridization of Probe
91
Chapter 13 Transformation of Saccharomyces cerevisiae
103
Chapter 14 Transformation of Escherichia coli by Plasmid DNA
109
Chapter 15 Protein Assays
115
Chapter 16 βGalaetosidase Assay
121
Chapter 17 Determination of βGalactosidase in Permeabilized Yeast Cells
125
Chapter 18 Assay of βGalactosidase in Cell Extracts
129
Appendix 8 Storage of Cultures
182
Appendix 9 Sterilization Methods
184
Appendix 10 Preparation of Stock Solutions for Culture Media
186
Appendix 11 Growth in Liquid Medium
188
Appendix 12 Determination of Viable Cells
191
Appendix 13 Determination of Cell Mass
193
Appendix 14 Determination of Cell Number
195
Appendix 15 Nomenclature of Strains
198
Appendix 16 Glassware and Plasticware
201
Appendix 17 Preparation of Tris and EDTA
203
Appendix 18 Basic Rules for Handling Enzymes
206
Appendix 19 Manufacturers and Distributors Addresses
210
Glossary
217
Index
223
Copyright

Other editions - View all

Common terms and phrases

Bibliographic information