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Establishing a Plant Cell Culture
Preparation and Fusion of Protoplasts
Regeneration and Cloning
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acid agarose agglutination Agrobacterium tumefaciens albumin antigen antiserum assay autoclaved bacterium binding buffer Callus cell culture cell lines cell pellet cell suspension cell wall centrifuge tube chloroplasts color concentration Conjugate containing continued destain detection dilution distilled water enzyme erythrocytes ethanol experiment Experimental Outline explants flask fluorescent forceps g/ml genetic genome goat HBSS homogenate immunoassays immunoglobulin Incubate lab period labeled laboratory layer lectin lymphocytes lymphoid Mark Holland medium Method minutes mitochondria ml pipets Module molecules mouse number of cells paper clips Pasteur pipets Pasteur pipette PBS-BSA petridish pipet Plant Cell Culture plant material plant tissue plant tissue culture Pre-lab Preparation precipitate precipitin lines procedure proteins protocol protoplasts rabbit serum receptors region remove Results Introduction resuspend Safety Guidelines sample seedlings solution spectrophotometer spleen cells stain sterile substrate sucrose supernatant surface test tube tissue culture transfer trypan blue trypsin tumor wash water bath