Biotechnology: Genetic fundamentals and genetic engineering
Without sufficient knowledge of classical and molecular genetics as well as of genetic engineering, it is not possible to fully unterstand biotechnological processes. Clear, concise and comprehensive, Volume 2 gives scientists all the information they need to carry out research and production in this field.
In Part I of the book the classical genetic techniques relevant to biotechnology are presented. Part II highlights the recent advances in molecular genetics which have revolutionized our knowledge in the field. Part III deals with the genetic engineering of microorganisms, and Parts IV and V concentrate on plants and animals - multicellular organsims which require special genetic engineering techniques. In the final Part VI biosafety concepts in modern biotechnology are treated.
Topics included are: Mutagenesis/ Genetic Exchange Processes/ Cell Fusion/ Gene Mapping/ Transposable Elements/ Gene Expression/ Synthesis of Oligonucleotides/ Polymerase Chain Reaction (PCR)/ Transposons/ Transformation Vectors/ Recombinant Proteins/ Gene Amplification/ Transgenic Plants and Animals/ Biosafety Concepts
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Bruce W Holloway
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Acad activity Agrobacterium Agrobacterium tumefaciens amino acid amplification analysis animals antibiotic antisense Bacillus bacteria Bacteriol bacteriophage binding Biol cDNA chemical chromosome cloning codon construct containing detection DNA fragments DNA polymerase DNA sequencing double-stranded efficient EMBO encoding enzyme Escherichia coli eukaryotic factors formation function fungi gene expression gene transfer genome homologous hybridization induced insertion integration isolated mammalian mapping membrane ments method mice Microbiol molecular molecules mRNA mutagenesis mutagenic mutation Natl nested sets nuclear Nucleic Acids Nucleic Acids Res nucleotide oligonucleotides phage plant cells plasmid primer Proc production promoter protein protoplast fusion protoplasts quence reaction recombination region replication resistance restriction ribosomal Sect selectable marker single-stranded sion specific strains strand strategy Streptomyces structure subtilis supercoiling synthesis T-DNA target techniques tion tobacco trans transcription transformation transgenic plants translation transposon vectors viral virus viruses vitro vivo yeast Z-DNA