Biothermodynamics, Part 3

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Melvin I. Simon, Michael L. Johnson, Jo M. Holt, Gary K. Ackers
Academic Press, Jan 26, 2011 - Medical - 401 pages

In the past several years, there has been an explosion in the ability of biologists, molecular biologists and biochemists to collect vast amounts of data on their systems. Biothermodynamics, Part C presents sophisticated methods for estimating the thermodynamic parameters of specific protein-protein, protein-DNA and small molecule interactions.

The use of thermodynamics in biological research is used as an "energy book-keeping system.” While the structure and function of a molecule is important, it is equally important to know what drives the energy force. These methods look to answer: What are the sources of energy that drive the function? Which of the pathways are of biological significance?

As the base of macromolecular structures continues to expand through powerful techniques of molecular biology, such as X-ray crystal data and spectroscopy methods, the importance of tested and reliable methods for answering these questions will continue to expand as well.



  • Elucidates the relationships between structure and energetics and their applications to molecular design, aiding researchers in the design of medically important molecules
  • Provides a "must-have" methods volume that keeps MIE buyers and online subscribers up-to-date with the latest research
  • Offers step-by-step lab instructions, including necessary equipment, from a global research community
 

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Contents

Measurement and Analysis of Equilibrium Binding Titrations A Beginners Guide
1
Macromolecular Competition Titration Method Accessing Thermodynamics of the Unmodified MacromoleculeLigand Interactions Through Spectros...
17
Analysis of PKRRNA Interactions by Sedimentation Velocity
59
Structural and Thermodynamic Analysis of PDZLigand Interactions
81
Thermodynamic Analysis of Metal IonInduced Protein Assembly
101
Thermodynamic Dissection of Colicin Interactions
123
Energetics of Src Homology Domain Interactions in Receptor Tyrosine KinaseMediated Signaling
147
Structural and Functional Energetic Linkages in Allosteric Regulation of Muscle Pyruvate Kinase
185
Analysis of Free Energy Versus Temperature Curves in Protein Folding and Macromolecular Interactions
219
Application of the Sequential nStep Kinetic Mechanism to Polypeptide Translocases
239
A Coupled Equilibrium Approach to Study Nucleosome Thermodynamics
265
Quantitative Methods for Measuring DNA Flexibility In Vitro and In Vivo
287
Author Index
337
Subject Index
349
Color Plates
355
Copyright

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