Bone Research Protocols

Front Cover
Miep H. Helfrich, Stuart Ralston
Springer Science & Business Media, 2003 - Medical - 448 pages
A collection of the latest laboratory techniques for the study of bone and bone tissue. Described in step-by-step detail, these readily reproducible methods cover such topics as the isolation and culture of bone cells, the preparation of bone tissue for histological and ultrastructural analysis, methods for the measurement of bone strength and for mechanical studies, and how to use digital imaging techniques in the analysis of bone.
 

What people are saying - Write a review

We haven't found any reviews in the usual places.

Contents

Osteoblast Isolation from Murine Calvariae and Long Bones
19
Mineralizing FibroblastColonyForming Assays
29
Osteocyte Isolation and Culture
41
CULTURE OF OSTEOCLASTS
53
Isolation and Purification of Rabbit Osteoclasts
89
Generating Human Osteoclasts from Peripheral Blood
101
Generating Murine Osteoclasts from Bone Marrow
129
Osteoclast Formation in the Mouse Coculture Assay
145
Fluorescence Imaging of BoneResorbing Osteoclasts
259
IMAGING TECHNIQUES
261
Bone Histomorphometry
283
Transmission Electron Microscopy of Bone
299
Scanning Electron Microscopy of Bone
311
Bone Measurements by Peripheral Quantitative
323
The IMO Model
353
Ovariectomy and Estrogen Replacement in Rodents
361

RANKLMediated Osteoclast Formation
153
Analysis of Osteoclast Function in Mouse Calvarial Cultures
169
Assessing Bone Formation
183
HISTOLOGICAL AND ULTRASTRUCTURAL TECHNIQUES
199
Techniques for the Study of Apoptosis in Bone
225
Protein Localization in WaxEmbedded and Frozen Sections
237
Detection of Noncollagenous Bone Proteins
249
Mechanical Testing of Bone Ex Vivo
369
Bone Cell Responses to Fluid Flow
381
Methods for Analyzing Bone Cell Responses to Mechanical
399
Extraction of Nucleic Acids from Bone
425
Analysis of Gene Expression in Bone by Quantitative RTPCR
433
Index
441
Copyright

Other editions - View all

Common terms and phrases

Bibliographic information