Bone Marrow and Stem Cell Transplantation

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Meral Beksaç
Springer Science & Business Media, May 3, 2007 - Medical - 234 pages
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This volume is a compendium of cutting-edge molecular methods for the successful transplantation of hematopoietic stem cells. The availability of genetic profiles for committed and uncommitted stem cells combined with the determination of molecular steps involved in hematopoietic stem cell activation and self-renewal hold tremendous promise for future success in the clinic. Currently, stem cells can be quantified and sorted successfully, but transduction, labeling, and in vivo tracking of stem cells are difficult techniques with traditionally low efficiency levels. The contributors to this volume describe promising tools for stem cell transplant research models, such as in vivo bioluminescence imaging.


Topics included in this volume are molecular profiling of hematopoietic stell cells, methods for imaging cell fates in hematopoiesis, PCR-SSP typing of HLA Class I and Class II alleles, sequence-based HLA typing, and typing minor HLA antigens. Also discussed in this volume are the roles of natural killer cells and killer inhibitor receptor polymorphisms, polymorphisms within epithelial receptors, molecular methods used for the detection of autologous graft contamination in lymphoid disorders, and the application of proteomics to posttransplantation follow-up. With the recent trend moving from gene expression to the protein expression profiles, the application of proteomics to the posttransplant follow-up of patients is becoming routine. As a result, this volume will become an invaluable source for biochemists, molecular biologists, and clinicians.

 

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Contents

Molecular Profiling of Hematopoietic Stem Cells
1
Methods for Imaging Cell Fates in Hematopoiesis
17
An Overview of HLA Typing for Hematopoietic Stem Cell Transplantation
35
SequenceSpecific Primed PCR PCRSSP Typing of HLA Class I and Class II Alleles
51
HLA Typing with SequenceSpecific Oligonucleotide Primed PCR PCRSSO and Use of the Luminex Technology
61
SequencingBased Typing of HLA
71
Molecular Typing Methods for Minor Histocompatibility Antigens
81
NonHLA Gene Polymorphisms in Stem Cell Transplantation
97
Role of Natural Killer Cells and Killer ImmunoglobulinLike Receptor Polymorphisms Association of HLA and KIRs
123
Identification of Bone Marrow Derived Nonhematopoietic Cells by Double Labeling with Immunohistochemistry and In Situ Hybridization
145
Molecular Methods Used for Detection of Minimal Residual Disease Following Hematopoietic Stem Cell Transplantation in Myeloid Disorders
161
Molecular Methods Used for The Detection of Autologous Graft Contamination in Lymphoid Disorders
179
Detection of Impending Graft Rejection and Relapse by LineageSpecific Chimerism Analysis
197
Application of Proteomics to Posttransplantational FollowUp
217
Index
229
Copyright

Polymorphisms Within Epithelial Receptors NOD2CARD15
115

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