C. Elegans: Methods and Applications
Springer Science & Business Media, 2006 - Science - 292 pages
The goal of C. elegans is to provide overviews and detailed step-by-step descriptions of newer and state-of-the-art methods utilized in the field. These include tools essential for forward and reverse genetic analysis, data mining, and comparative genomics strategies, electron and fluorescence microscopy methods, automated imaging methods for worm behavioral analysis, functional genomics strategies, and methods for physiological analyses including somatic cell culture, toxicity assays, electrophysiology, and in vivo imaging of intracellular Ca2+ and pH using genetically encoded fluorescent indicator proteins.
What people are saying - Write a review
We haven't found any reviews in the usual places.
Other editions - View all
AceDB agar agarose alignments alleles allow analysis animals antibody approx assay bacteria behavior Biol Biology briggsae Caenorhabditis elegans calcium camera cell centrifuge chromosome clone coli containing cover slip Cry5B culture database detection dsRNA edited eggs elegans genes embryo encodes enzyme filter freeze chamber freeze substitution function g/mL gene expression genetic genotype gonad green fluorescent protein hermaphrodites identified Incubate insertion introns M9 buffer mapping marker membrane Methods microarray microparticle bombardment microscope Molecular Mos1 muscle mutagenesis mutagenized mutant nematode nematode Caenorhabditis elegans neurons Note oocytes orthologs pellet phenotype pHluorin pipet plasmid plates primers progeny Protocols ratio receptor recombinants region RNA interference RNAi screen script signal solution specific sperm sterile strains Subheading synaptic syntenic syntenic blocks techniques temperature template tion toxin transcription transfer transgenic transposon tube variants vitro transcription wash wild-type WormBase worms