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Biochemical properties of the SR Ca2channel protein from
The biochemical basis for PSE quality problems associated with
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3H]ryanodine binding 75 kDa protein affinity labeling Airey autoradiogram avian binding of CaM Ca2*-channel protein activity Ca2*-channel protein isoforms CaCl2 caffeine Calmodulin calsequestrin CaM affinity labeling CaM binding CaM kinase CaM molecules cardiac Ca2*-channel protein cardiac muscle channel protein commercial and unimproved commercial turkey commercial turkey heavy commercial turkey skeletal crude dependence of 3H]ryanodine DHP receptor EGTA excitation-contraction coupling Figure fluorescence anisotropy fraction free Ca2 function heavy SR preparations heavy SR vesicles Hepes indicated inhibited lanes ligand mammalian skeletal muscle Meissner and Henderson membrane MgCl2 Mickelson muscle Ca2*-channel protein NaCl Pessah phosphorylation pmol/mg presence of EGTA protein isoforms PSE meat purified cardiac Ca2*-channel results suggest Rh-CaM Ryanodine Receptor sample Sarcoplasmic Reticulum Scatchard plot SDS-PAGE skeletal Ca2*-channel protein skeletal heavy skeletal muscle Ca2*-channel skeletal muscle heavy Stains-all Strasburg sucrose T-tubule terminal cistemae tetramer titration turkey skeletal muscle unimproved and commercial wheat germ wheat germ CaM