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40 mM K-oxalate accumulate Ca2+ axons Baumann and Walz Berridge birefringent Ca-oxalate deposits Ca-oxalate formation Ca-oxalate loaded Ca-oxalate precipitates Ca2+ gradient Ca2+ ions Ca2+ pump Ca2+-release Ca2+-sequestering organelles calcium calcium content cell stimulation changes in Ca2+ cisternae cryosections Cytochemistry cytoplasm cytosol digitonin electron dense electron microscopy electron probe X-ray endomembrane system endoplasmic reticulum EPMA Fabiato free Ca2+ concentration freeze substituted function Golgi apparatus honeybee honeybee drone incubated inositol intracellular topography leech photoreceptor Limulus loading medium containing localization of Ca2+-sequestering measured mechanisms methodological MgATP micrographs mitochondria mitochondrial granules mmol/kg dry wt muscle cells neuronal non-muscle cells obtained optical signal organelles Os04-impregnation OsFeCN oxalate concentrations oxalate method oxalate2 Payne photoreceptor cells Physiol plasma membrane polarization postfixed precipitating anion prepared for electron probe X-ray microanalysis release Ca2+ retina saponin sarcoplasmic reticulum shock frozen smooth muscle Somlyo spatial specific staining specimen spectra structural studies Teil tion tissue slices X-ray