Calculations in Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory

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Academic Press, 2003 - Mathematics - 302 pages
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Calculations in Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory is the first comprehensive guide devoted exclusively to calculations encountered in the genetic engineering laboratory. Mathematics, as a vital component of the successful design and interpretation of basic research, is used daily in laboratory work. This guide, written for students, technicians, and scientists, provides example calculations for the most frequently confronted problems encountered in gene discovery and analysis. The text and sample calculations are written in an easy-to-follow format. It is the perfect laboratory companion for anyone working in DNA manipulation and analysis.

*A comprehensive guide to calculations for a wide variety of problems encountered in the basic research laboratory.
* Example calculations are worked through from start to finish in easy-to-follow steps
* Key chapters devoted to calculations encountered when working with bacteria, phage, PCR, radioisotopes, recombinant DNA, centrifugation, oligonucleotides, protein, and forensic science.
*Written for students and laboratory technicians but a useful reference for the more experienced researcher.
*A valuable teaching resource.
 

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Contents

Chapter 1 Scientific Notation and Metric Prefixes
1
Chapter 2 Solutions Mixtures and Media
18
Chapter 3 Cell Growth
42
Chapter 4 Working with Bacteriophage
77
Chapter 5 Quantitation of Nucleic Acids
90
Chapter 6 Labeling Nucleic Acids with Radioisotopes
109
Chapter 7 Oligonucleotide Synthesis
136
Chapter 8 The Polymerase Chain Reaction
143
Chapter 9 Recombinant DNA
186
Chapter 10 Protein
242
Chapter 11 Centrifugation
270
Chapter 12 Forensic Science
278
Index
297
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About the author (2003)

Frank Stephenson received his doctorate in molecular biology from UC Berkeley and has published several books in the field including 'DNA: How the Biotech Revolution is Changing the Way We Fight Disease' and 'A Hands-On Introduction to Forensic Science: Cracking the Case'. He is currently an instructor in the Technical Training Department with ThermoFisher Scientific, the world's leading manufacturer of instrumentation and reagents for the biotechnology industry.

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