## Calculations in Molecular Biology and Biotechnology: A Guide to Mathematics in the LaboratoryCalculations in Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory is the first comprehensive guide devoted exclusively to calculations encountered in the genetic engineering laboratory. Mathematics, as a vital component of the successful design and interpretation of basic research, is used daily in laboratory work. This guide, written for students, technicians, and scientists, provides example calculations for the most frequently confronted problems encountered in gene discovery and analysis. The text and sample calculations are written in an easy-to-follow format. It is the perfect laboratory companion for anyone working in DNA manipulation and analysis.*A comprehensive guide to calculations for a wide variety of problems encountered in the basic research laboratory. * Example calculations are worked through from start to finish in easy-to-follow steps * Key chapters devoted to calculations encountered when working with bacteria, phage, PCR, radioisotopes, recombinant DNA, centrifugation, oligonucleotides, protein, and forensic science. *Written for students and laboratory technicians but a useful reference for the more experienced researcher. *A valuable teaching resource. |

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### Contents

Chapter 1 Scientific Notation and Metric Prefixes | 1 |

Chapter 2 Solutions Mixtures and Media | 18 |

Chapter 3 Cell Growth | 42 |

Chapter 4 Working with Bacteriophage | 77 |

Chapter 5 Quantitation of Nucleic Acids | 90 |

Chapter 6 Labeling Nucleic Acids with Radioisotopes | 109 |

Chapter 7 Oligonucleotide Synthesis | 136 |

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### Common terms and phrases

absorbance added amount antilog assay axis B-galactosidase bacteriophage base pairs buffer calculated as follows cDNA cell concentration cells/mL chloramphenicol cloning conversion factor converted copies culture decimal determine dGTP dilution factor DNA fragment DNA polymerase dNTP ends/mL enzyme equivalent exponent expressed extinction coefficient following equation following relationship following result g/mol gene genome genotype frequencies gives the following grams hybridization infected cells insert labeled lambda ligation reaction linear logarithm mg/mL molar molecular weight molecules moles multiplied mutation NaCl nick translation nmol nucleic acid nucleotides oligonucleotide PCR reaction phage picomoles plasmid plate plot pmol Poisson distribution primer probe Problem protein quantity reaction contains recombinant recombinant clones regression line scientific notation second strand semilog graph sequence significant digits Solving specific activity stock solution strand cDNA synthesis target template total cpm total number tube vector