Capillary Electrophoresis: Theory and Practice
Paul D. Grossman, Joel C. Colburn
Academic Press, Dec 2, 2012 - Science - 352 pages
This book is designed to be a practical guide, used by wide audience, including those new to CE, those more experienced, routine users, those interested in technology development, and those involved with applications research. References have been emphasized to allow the reader to explore the detailed specifics and theoretical foundations.
This book draws together the rapidly evolving, diverse, and multidisciplinary subject of capillary electrophoresis (CE). It is designed as a practical guide to be used by a wide audience, including those new to CE as well as more experienced users. This volume presents the capabilities, limitations, potentials, and future challenges facing each area of CE. Key aspects of this technique, such as high resolution capability, full automation, high speed separations, quantification of nanoliter sample volumes, and simultaneous multiple detection capabilities are presented in a concise and logical fashion. This book is designed to help you make the most of your CE separations, and includes comprehensive information on:
Electroosmosis, separation efficiency, and Joule heating
In-depth discussion of the separation principles and capabilities of the major modes of CE
Sieving gel electrophoresis
Free solution CE
Micellar electrokinetic capillary chromatography
Entangled polymer matrix-based separation
Detailed treatment of the application of CE to a wide range of molecules, supplemented with extensive "hands-on" illustrations
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amino acids ampholytes Anal analysis analyte anions applied Balchunas capillary diameter capillary electrophoresis capillary length capillary wall cationic charge Chem chiral Chromatogr chromatography cm to detector coefﬁcient concentration Conﬁrm convection deﬁned derivatization detection limits difﬁcult diffusion effect efﬁciency elec electrical ﬁeld strength electrode electrokinetic electroosmotic ﬂow electropherogram electrophoresis capillary electrophoretic mobility elution range ﬁber Figure ﬁrst ﬂow fused-silica gel electrophoresis gel-ﬁlled capillary Grossman Hjertén HPCE HPLC hydrophobic i.d. capillary increase inﬂuence injection interactions ionic isoelectric focusing Jorgenson Joule heating Karger laser linear MECC method micellar micelle migration mobile phase molecular molecules myoglobin neutral Nishi oligonucleotides optical organic modiﬁer parameters peak peptides phosphate polymer proﬁle proteins quantitative Reproduced with permission resolution result sample sensitivity Sepaniak separation signiﬁcant sodium solution solvent speciﬁc sufﬁcient surfactants technique temperature Terabe theoretical plates tion tricine tube UV-absorbance V/cm velocity voltage volume Wallingford wavelength zone