Capillary Electrophoresis and Microchip Capillary Electrophoresis: Principles, Applications, and Limitations

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John Wiley & Sons, Feb 1, 2013 - Science - 416 pages
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Explores the benefits and limitations of the latest capillary electrophoresis techniques

Capillary electrophoresis and microchip capillary electrophoresis are powerful analytical tools that are particularly suited for separating and analyzing biomolecules. In comparison with traditional analytical techniques, capillary electrophoresis and microchip capillary electrophoresis offer the benefits of speed, small sample and solvent consumption, low cost, and the possibility of miniaturization.

With contributions from a team of leading analytical scientists, Capillary Electrophoresis and Microchip Capillary Electrophoresis explains how researchers can take full advantage of all the latest techniques, emphasizing applications in which capillary electrophoresis has proven superiority over other analytical approaches. The authors not only explore the benefits of each technique, but also the limitations, enabling readers to choose the most appropriate technique to analyze a particular sample.

The book's twenty-one chapters explore fundamental aspects of electrophoretically driven separations, instrumentation, sampling techniques, separation modes, detection systems, optimization strategies for method development, and applications. Specific topics include:

  • Critical evaluation of the use of surfactants in capillary electrophoresis
  • Sampling and quantitative analysis in capillary electrophoresis
  • Capillary electrophoresis with electrochemical detection
  • Overcoming challenges in using microchip electrophoresis for extended monitoring applications
  • Capillary electrophoresis of intact unfractionated heparin and related impurities
  • Microchip capillary electrophoresis for in situ planetary exploration

Each chapter begins with an introduction and ends with conclusions as well as references to the primary literature. Novices to the field will find this book an easy-to-follow introduction to core capillary electrophoresis techniques and methods. More experienced investigators can turn to the book for troubleshooting tips and expert advice to guide them through the most advanced applications.

 

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Contents

Detection C4D Applied to Capillary Electrophoresis CE
xxiii
Sample Stacking A Versatile Approach for Analyte
xxvii
Sam Iin and uantitative Anal sis in Ca illar
xxxviii
Practical Considerations for the Design
4
Improving the Separation in Microchip
56
Capillary Electrophoretic Reactor and Microchip
84
Methods to
11
Capillary Electrophoresis with Electrochemical
39
Capillary Electrophoresis of Intact Unfractionated
2009
Microchip Capillary Electrophoresis for IN STU
2009
Rapid Analysis of Charge Heterogeneity
2009
Application of Capillary Electrophoresis for High
2009
Electrokinetic Transport of Microparticles in
2009
Integration of Nanomaterials in Capillary
2009
Microchip Capillary Electrophoresis to Study
2015
Glycomic Profiling Through Capillary
2029

Electrophoresis
90
Distinction of Coexisting Protein Conformations
91
Capillary Electromigration Techniques for
45
Capillary Electrophoresis and Multicolor
81
Index
2060
Capacitively Coupled Contactless Conductivity
2063
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About the author (2013)

Carlos D. García, PhD, is an Associate Professor of Analytical Chemistry at the University of Texas at San Antonio, USA. His group is currently focused on the development of novel bioanalytical strategies involving microfluidics and nanomaterials.

Karin Y. Chumbimuni-Torres, PhD, is a Research Associate at the University of Texas at San Antonio, USA. She is interested in pursuing the development of electrochemical biosensors and their integration to microchip-based platforms.

Emanuel Carrilho, PhD, is an Associate Professor at the University of Säo Paulo, Brazil. With more than twenty-five years of experience in separation science, his group is focused on the development of analytical methods and instrumentation for bioanalyses.

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