Carnegie Institution of Washington Publication, Issue 271

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Carnegie Institution of Washington, 1918
 

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Page 144 - ... phosphatides. — Although the fats have been discussed before the phosphatides because of the simple structure of the former, were we to place them in the order of their importance in the production of vital phenomena, there can be little doubt that the phosphatides should have been considered first, as among the most important substances in living matter. For they are found in all cells, and it is undoubtedly their function to produce, with cholesterol, the peculiar semifluid, semisolid state...
Page 60 - ... with the stain and heat over a spirit lamp until fumes, smelling strongly of aniline oil, come off; allow to cool; let the stain remain on the sections about six minutes; return the stain to the bottle. (9...
Page 83 - ... of the unity of the class of granules under consideration. The evidence is briefly this: (1) Mitochondria are almost completely soluble in alcohol, chloroform, ether, and dilute acetic acid. They are rendered insoluble by chromization. They are not doubly refractile and they do not stain with either Sudan III or Scharlach R. They are only sometimes blackened with osmic acid. (2) It is said that part of the mitochondrial substance is not soluble in these fat solvents and it is supposed that this...
Page 82 - ... points out that their presence in large numbers is associated with intense protoplasmic activity. They are especially numerous in the active stages of cell life and diminish with senility, in both plants and animals. There is a sharp increase with regenerative activity and in compensatory hypertrophy. In the second place, there is a distinct reciprocal relationship between the amount of mitochondria and the amount of fat, which suggests some connection between the mitochondria and oxidation....
Page 157 - Organismen 34:287-293. 1914. Chromosomal variations and the formation of the first spermatocyte chromosomes in the European Earwig, Forficula sp.
Page 155 - Mitochondria (and other cytoplasmic structures) in tissue cultures," Amer. Journ. Anat., Vol. 17, p. 339. LEWIS, MR, AND ROBERTSON, WRB 1916 "The Mitochondria and other structures observed by the tissue culture method in the male germ cells of Chorthippus curtipennis, scudd,
Page 63 - Phosphotungstic acid, i gm.: aq. dest., 100 cc; brazilin, 0.05 gm. The brazilin is first dissolved in a small quantity of distilled water by the aid of heat and added to the phosphotungstic acid solution. Ripening may be accelerated by the addition of 0.4 cc of hydrogen peroxide, or of a few drops of a solution of soluble molybdic acid. The solution deteriorates with age and should not be used after three days.
Page 155 - LILLIE, RS 1913 The formation of indophenol at the nuclear and plasma membranes of frog's blood corpuscles and its acceleration by induction shocks. Jour. Biol. Chem., vol. 15, p. 237-248, 1 PI. MAST, SO 1911 Light and the Behavior of Organisms, xi + 410 p., 36 figs., New York. MINES, GR 1912 Note on the mechanism of discharge of the Cuvierian organs of Holothuria nigra. Quart. Jour. Micr. Sci., vol. 57, p. 301-304, 1 PI.
Page 149 - Spermatid transformations in Gryllus assimilis, with special reference to the Nebenkern, [with PI.
Page 60 - The stain should be ready to use in about twenty-four hours. It goes bad in about a month. To stain, dry the slide with a towel, except the small area to which the sections are attached; cover the sections...

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