Cell Biological Applications of Confocal Microscopy

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Academic Press, Nov 17, 1993 - Science - 380 pages
Cell Biological Applications of Confocal Microscopy instructs researchers on methods of preparing biological material for confocal microscopy. Individual chapters describe the technical problems of imaging structures in thick specimens and useful techniques such as multi-color dye visualization. The book provides specific examples of applications of confocal microscopy to cell biological problems. This volume is an essential guide for students and scientists incell biology, neuroscience, and many other areas of biological and biomedical research, as well as research directors and technical staff of microscopy and imaging facilities.

Describes proper specimen preparation for obtaining high-quality data and images
Covers pitfalls in imaging different specimens and in employing different dyes
Contains detailed protocols accessible to new users
Chapters are self-contained units, but describe strategies that can be combined to develop new protocols
Techniques are illustrated with full-color plates
 

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Contents

Chapter 1 An Introduction to Confocal Microscopy and ThreeDimensional Reconstruction
1
Chapter 2 Rapid Scanning Confocal Microscopy
47
Important Considerations for Accurate Imaging
79
Practical Application and Limitations
97
Chapter 5 Measurement of Intracellular pH with a Laser Scanning Confocal Microscope
183
Chapter 6 Confocal Microscopy of Potentiometric Fluorescent Dyes
195
Chapter 7 Imaging Endoplasmic Reticulum in Living Sea Urchin Eggs
211
Assessment by Confocal Microscopy
221
Chapter 9 Confocal Immunofluorescence Microscopy of Microtubules in Amphibian Oocytes and Eggs
241
Chapter 10 Applications of Confocal Microscopy to Studies of Sea Urchin Embryogenesis
265
Immunohistochemical Preparation and Fluorescence Confocal Microscopy
289
Chapter 12 Using Confocal Laser Scanning Microscopy to Investigate the Organization and Development of Neuronal Projections Labeled with DiI
325
Fluorescent and Reflection Stains
345
Index
367
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About the author (1993)

Dr. Brian Matsumoto is a retired research scientist who has worked for 30 years documenting experiments with technologically advanced cameras. He now spends his time photographing with a variety of cameras and lenses, and is interested in expanding a camera s potential by pairing it with specialized optics such as microscopes and telescopes. He enjoys spending time outdoors and carries his camera on all of his hikes to photograph nature. In addition to the nine books he has written for Rocky Nook, Dr. Matsumoto has published several articles and has had his photographs published in a number of periodicals. His photographs have also appeared on the covers of American Laboratory, BioTechniques, and BioPhotonics. He is experienced in the technical aspects of photography and is an adjunct instructor for the Hooke College of Applied Sciences. Dr. Matsumoto recently served as a judge for the Olympus BioScapes International Digital Imaging Competition.

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