The present title Cell Culture is an attempt to present an account of what is known and what is postulatea about the cell culture, and the subsequent advancement in the field. Certain basic scientific facts are included, it is true, but the majority of the contents are based upon the clinical work.
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Animal Tissue Culture
Techniques of Animal Cell Culture
Physical Methods of Cell Separation
Prevention of Contamination
Cell Culture in Plants
Maintenance of a Cell Line
Formation of Protoplasts
Cloning of Cells
acid adult tissues agar alcohol animal cells antibiotics ascites aseptic technique autoclave balanced salt solution blood bottle bovine caps cell culture cell lines cell suspension cell types centrifuge tubes chamber chick clean cloning collagen collagenase concentration containing count coverslip culture flasks culture medium cultured cells density detergent developed differentiation diluted disaggregation dry heat embryo embryo extract enzyme epithelial cells epithelium explants fibroblasts filter fluid forceps fragments glass glassware gradient grow growth medium human incubator inoculated laboratory laminar flow layer liver material melanocyte membrane method microscope mixture monolayer normal nutrients obtained organ culture Pasteur pipette petri dishes placed plant tissue plant tissue culture plastic plate prepared primary culture procedure protein Protocol protoplasts removed Resuspend rinse room temperature scalpel sedimentation separation serum silicone single cell slide sodium sterile stock solution streptomycin subculture supernatant surface syringe tissue culture transfer trypsin tumour vessels vitro vivo washing