Cell Culture in the Neurosciences
Jane Bottenstein, Gordon Sato
Springer US, 1985 - Medical - 383 pages
A fundamental problem in neuroscience is the elucidation of the cellular and molecular mechanisms underlying the development and function of the nervous system. The complexity of organization, the heteroge neity of cell types and their interactions, and the difficulty of controlling experimental variables in intact organisms make this a formidable task. Because of the ability that it affords to analyze smaller components of the nervous system (even single cells in some cases) and to better control experimental variables, cell culture has become an increasingly valuable tool for neuroscientists. Many aspects of neural development, such as proliferation, differentiation, synaptogenesis, and myelination, occur in culture with time courses remarkably similar to those in vivo. Thus, in vitro methods often provide excellent model systems for investigating neurobiological questions. Ross Harrison described the first culture of neural tissue in 1907 and used morphological methods to analyze the cultures. Since that time the technique has been progressively modified and used to address an ever widening range of developmental questions. In recent years a con vergence of new or improved cell culture, biochemical, electrophysiol ogical, and immunological methods has occurred and been brought to bear on neurobiological questions. This volume is intended not to be comprehensive but rather to highlight some of the latest findings, with a review of previous important work as well, in which combinations of these methods are used.
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Growth and Differentiation of Neural Cells in Defined
SerumFree Defined Media for Neural Precursor Cells Conclusion
Neuronal and Glial Surface Antigens on Cells in Culture
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Acad acid activity adrenal adrenergic aggregates antigen assay astrocytes axonal binding biochemical Bottenstein brain cells Brain Res Caš catecholamines Cell Biol cell cultures cell line cell surface cell types cellular cerebellar channel chick cholinergic clonal defined medium developmental differentiation dissociated effect embryonic enzyme explants extracellular fibroblasts fibronectin function ganglia ganglion ganglioside glial cells glycoprotein GPDH Honegger hormones increase induced insulin marker membrane molecules monoclonal antibody morphological mouse myelin N-CAM Natl nerve growth factor nervous system neural cells neural crest cells neurite neurite outgrowth neuroblastoma neuroblastoma cells Neurochem neuronotrophic neurons Neurosci neurotransmitter oligodendrocytes Patterson PC12 cells Perez-Polo pheochromocytoma pheochromocytoma cells primary cultures Proc proliferation properties protein purified rat brain receptors response retinal Schachner Schwann cells serum serum-free Sieber-Blum specific spinal cord stimulation studies surface antigens survival sympathetic neurons synaptic synthesis tetanus toxin Thoenen tissue unpigmented Vellis vitro vivo
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