Cellular Neurobiology: A Practical Approach
John Chad, H. V. Wheal
IRL Press at Oxford University Press, 1991 - Cells, Cultured. - 287 pages
Cellular Neurobiology covers techniques from basic in vitro maintenance of cells, through perfusion and recording methods, to advanced topics such as optical imaging of ionic activity and mathematical modelling of the properties of excitable membranes.
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Culture of adult mammalian peripheral
Dissociated neurons from adult
Production of the organoptypic slice
Applications of slice cultures produced by
Cell culture procedures for the extension of neurites
NG 10815 neuroblastoma x glioma hybrid
Separation of sodium and calcium membrane currents
Wholecell voltageclamp techniques
Photolabile calcium buffers to selectively
Caged molecules activated by light
Electrical behaviour of NG 10815 cells
Putting theory into practice
Perfusion of nerve cells and separation
Functional modelling of neuronal and dendritic function
Deriving channel reversal potential for simulations
why model single cells?
ACSF action potentials activation adult rat DRG axon BAPTA block blockers brain Ca-activated caged compounds caged phenylephrine calcium buffers calcium channels calcium concentration calcium currents calcium imaging camera camera lucida cellular chamber chemical chemical synapses command potential conductance coverslips culture dish dendritic depolarization dissecting dissociation DM-nitrophen electrical electrode enzyme experimental experiments fibre fluorescence function ganglia gating particle haemolymph hippocampal HPLC inactivation incubation InsP3 ionic ions isolated Journal of Neuroscience Journal of Physiology kinetics lamp light measured medium membrane potential method microscope molecules msec muscle neurites Neuroscience nucleotides obtained optical outward current parameters pasteur pipette perfusion phenylephrine photolabile photolysis Physiology London plated potassium preparation probes processes properties Protocol recording reversal potential sections shown in Figure slices sodium somata staining steady-state studies superfusate synaptic tail current techniques time-constant tion tissue culture tube voltage clamp voltage-dependent wavelengths whole-cell
Page 273 - This research is sponsored by a grant from the Office of Naval Research (ONR), Cognitive and Neural Sciences Division; by the Alfred P. Sloan Foundation; by the National Science Foundation; by the Artificial Intelligence Center of Hughes Aircraft Corporation (Sl-8015342); by EI DuPont DeNemours and Company; and by the NATO Scientific Affairs Division (0403/87).
Page 274 - Single channel currents recorded from membrane of denervated frog muscle fibers. Nature 260:799-801.
Page 273 - This paper describes research done within the Center for Biological Information Processing, in the Department of Brain and Cognitive Sciences, and at the Artificial Intelligence Laboratory.