Cellular Neurobiology: A Practical Approach

Front Cover
John Chad, H. V. Wheal
IRL Press at Oxford University Press, 1991 - Cells, Cultured. - 287 pages
Cellular Neurobiology covers techniques from basic in vitro maintenance of cells, through perfusion and recording methods, to advanced topics such as optical imaging of ionic activity and mathematical modelling of the properties of excitable membranes.
 

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Contents

Culture of adult mammalian peripheral
3
Discussion
12
Dissociated neurons from adult
19
Practical considerations
32
Production of the organoptypic slice
39
Applications of slice cultures produced by
52
Cell culture procedures for the extension of neurites
58
NG 10815 neuroblastoma x glioma hybrid
75
Separation of sodium and calcium membrane currents
127
Wholecell voltageclamp techniques
137
Slice chambers
140
Practical aspects
146
Photolabile calcium buffers to selectively
153
Caged molecules activated by light
179
Concluding remarks
202
Threedimensional reconstructions
225

Electrical behaviour of NG 10815 cells
82
General remarks
92
Voltagedependence
100
Reversal potential
104
Putting theory into practice
114
Perfusion of nerve cells and separation
121
Functional modelling of neuronal and dendritic function
236
Discussion
243
considering Ca2
258
Deriving channel reversal potential for simulations
262
why model single cells?
271
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Page 273 - This research is sponsored by a grant from the Office of Naval Research (ONR), Cognitive and Neural Sciences Division; by the Alfred P. Sloan Foundation; by the National Science Foundation; by the Artificial Intelligence Center of Hughes Aircraft Corporation (Sl-8015342); by EI DuPont DeNemours and Company; and by the NATO Scientific Affairs Division (0403/87).
Page 273 - Support for the AI Laboratory's artificial intelligence research is provided by the Advanced Research Projects Agency of the Department of Defense under Army contract DACA76-85-C-0010, and in part by ONR contract N00014-85-K-0124.
Page 274 - Single channel currents recorded from membrane of denervated frog muscle fibers. Nature 260:799-801.
Page 273 - This paper describes research done within the Center for Biological Information Processing, in the Department of Brain and Cognitive Sciences, and at the Artificial Intelligence Laboratory.
Page 274 - The M-current and other potassium currents of vertebrate neurons. In Neuromodulation: The Biochemical Control of Neuronal Excitability (ed. LK Kaczmarek and IB Levitan).

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International Review of Cytology

Limited preview - 1994

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