Cellular and Molecular Methods in Neuroscience Research

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Adalberto Merighi, Giorgio Carmignoto
Springer Science & Business Media, Oct 1, 2002 - Medical - 303 pages
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Analysis of the neural tissue presents unique and peculiar technical problems encountered in everyday bench work. Numerous books dealing with cellular and molecular protocols for general use in cell biology are available, but few are specifically devoted exclusively to neurobiology. Moreover, the "cross-talk" between researchers with different backgrounds, i.e. histologists, cell and molecular biologists and physiologists, is still quite difficult, and very often one remains somehow "confined" to his or her own specific field of expertise never daring to explore "mysterious" lands unless having the support of a big laboratory beyond. The general idea beyond this project was to put together the contributions from a number of well-known neuroscientists to produce a book that offers a survey of the most updated techniques for the study of nerve cells. After a long time spent doing research in the neuroscience field, and having acquired a good technical background in certain specific fields of neurobiology we have realized how difficult is to be able to step into a different technology. This book endeavors to assist in that goal.
 

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Contents

Analyses of Intracellular Signal Transduction Pathways in CNS Progenitor Cells
1
Confocal and Electron Microscopic Tracking of Internalized Neuropeptides andor Their Receptors
15
Transfection Methods for Neurons in Primary Culture
29
Polyethylenimine A Versatile Cationic Polymer for PlasmidBased Gene Delivery in the CNS
37
Transfection of GABAA Receptor with GFPTagged Subunits in Neurons and HEK 293 Cells
53
Neuronal Transfection Using Particle Mediated Gene Transfer
67
Analysis of Gene Expression in Genetically Labeled Single Cells
85
Immunocytochemistry and In Situ Hybridization Their Combinations for Cytofunctional Approaches of Central and Peripheral Neurons
119
Immunocytochemical Labeling Methods and Related Techniques for Ultrastructural Analysis of Neuronal Connectivity
161
Combined Electrophysiological and Morphological Analyses of CNS Neurons
181
Tract Tracing Methods at the Light Microscopic Level
203
Tract Tracing Methods at the Ultrastructural Level
221
In Vivo Analysis of Cell Proliferation and Apoptosis in the CNS
235
Confocal Imaging of Nerve Cells and Their Connections
259
Confocal Imaging of Calcium Signaling in Cells from Acute Brain Slices
273
Index
285

In Situ Reverse Transcription PCR for Detection of mRNA in the CNS
145

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