Cellular calcium: a practical approach
IRL Press at Oxford University Press, 1991 - Antiques & Collectibles - 418 pages
Calcium is arguably the most diverse and widespread of the intracellular second messengers, being involved in many mechanisms of cellular biochemistry, physiology, and pathology, where it plays a key role in signal transduction. Hormones and other extracellular agents bring about many of their effects by causing changes intracellular calcium concentration. This book provides a comprehensive description of state-of-the-art techniques for measuring intracellular calcium concentrations, including protocols for the use of fluorescent, bioluminescent and metallochromic indicators, and various other intracellular probes. Also outlined are methods for controlling and changing the calcium ion concentration in cells, as well as procedures used to study the important proteins involved in the membrane transport and intracellular binding of calcium. All of the chapters provide expert practical guidance in the techniques described and list any problems likely to be encountered. This book will appeal to a wide range of biological and biomedical researchers, particularly those involved in cellular, molecular and neuro-biology.
What people are saying - Write a review
We haven't found any reviews in the usual places.
General concepts in the use of fluorescent Ca2+
Characteristics of fluorescent Ca2+ indicators
25 other sections not shown
Other editions - View all
acid aequorin affinity annexins approx assay ATPase autofluorescence Biochemical Biochemistry Biological Chemistry Ca2+ binding Ca2+ buffering CaCl2 calcium calibration calmodulin Carafoli cardiac Cell Biology cellular Centrifuge changes concentration containing cytoplasm cytosolic described detector diltiazem dilution EDTA EGTA electrode emission enzyme exchange activity excitation fibre Figure filter fluorescence fluorescent Ca2+ indicators free Ca2+ glass Homogenize increase incubation injection intracellular ion-selective ions Journal of Biological Journal of Physiology ligand light loading measurements medium membrane method Mg2+ microelectrode microinjection micropipette microscope microslide mitochondria mmol/kg d.w. Molecular msec myoplasm NaCl obtained pellet perfusion permeabilization phospholipid photolysis photomultiplier photon pipette plant cells potential preparation procedure protein Protocol pulse pump purified ratio reaction resealing resuspended sample sarcoplasmic reticulum Section signal solution STnCDANZ sucrose supernatant technique tissue Tsien tube vesicles washed wavelength