Centrifugation in biology and medical science
Presents a comprehensive account of the various roles that centrifugation takes in biomedical science and the diversity of instrumentation that is available to the researcher. Specific applications are discussed, noting advantages, limitations and other characteristics that are peculiar to each methodological approach. Deals with the historical evolution of the various centrifugal approaches so that recent developments can be appreciated in perspective.
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DENSITY GRADIENT CENTRIFUGATION IN ZONAL
SOME FUNDAMENTAL PHYSICAL
18 other sections not shown
acceleration Anal analytical analytical ultracentrifuge Anderson axial axis of rotation Beckman Instruments Biochem bottom cellular centrifugal force centrifuge tube Chapter components continuous-flow convection core Coriolis force CsCl deceleration dense density gradient centrifugation density gradient separations dient differential centrifugation diffusion distributor dxldt ellipsoid elutriation equation exponential gradients Ficoll fixed-angle rotors fractions gradient maker gradient solutes horizontal isokinetic isolation isopycnic separations linear liquid liver loading Ludox lysosomes medium metrizamide microsomes mitochondria mixing chamber molecular nuclei operation organelles particle sedimentation particle zones pellet Percoll peroxisomes Physical properties Pickels piston plasma membranes produced proteins pump radial radius rate separations refractive index relationship reograd ribosomes Rickwood rotor bowl rotor chamber rotor speed sample seal sedimentation coefficient sedimentation rates sedimenting particles Sheeler subcellular supernatant Svedberg swinging-bucket rotors Table tissue trifugation ultracentrifuge ultraspeed unloading values velocity vertical tube rotors viscosity volume w/w sucrose zonal centrifugation zonal rotors