Chromatin: A Practical Approach

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Hannah Gould
Oxford University Press, 1998 - Science - 231 pages
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Chromatin comprises the complex of nucleic acids and proteins that make up the chromosomes, and regulation of its structure is increasingly recognized as an important factor in gene expression. Researchers from related fields needing an introduction to the methodology of chromatin will find this book invaluable, as will experienced workers. Contents: Isolation and fractionation of chromatin and linker histones; Extraction and separation of core histones and non-histone chromosomal proteins; Mapping chromatin structure in Drosophila; In vivo footprint and chromatin analysis by LMPCR; Analysis of nuclear scaffold attachment regions; Electron microscopy of chromatin; Counting nucleosome cores on circular DNA using topoisomerase I; In vitro reconstitution of chromatin and analysis of nucleosome positioning; Transcriptional and structural analysis of chromatin assembled in vivo; An assembly of chromatin and nuclear structures in Xenopus egg extracts; Appendix: List of Suppliers; Index

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Contents

Isolation and sizefractionation of chromatin
11
Removal of linker histones from native chromatin
20
Characterization of the phosphorylation state of linker
27
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About the author (1998)

Hannah Gould, The Randall Institute, King's College, London.

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