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Cooperative Phenomena in the Adsorption of Phosphorylase Kinase and Phosphorylase
Hydrophobic Interaction Chromatography on NonCharged Sepharose Derivatives
Hydrophobic Interaction Chromatography on Phenyland OctylSepharose C14B
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0'Carra acid activity adenine adsorbed adsorption affinity chromatography albumin ammonium analogue antibodies antigens applied beads binding Biochem biological Biophys biospecific bound C.R. Lowe capacity cellulose Chem coenzyme column volume complex Compound CONCLUSIONS AND SUMMARY containing coupling crosslinked Cuatrecasas DEAE dextran DEAE-Sepharose derivatives desorbed dextran effect elution enzyme equilibrated extract Figure flow rate fractions gamma globulin gel filtration glucose glycogen phosphorylase gradient groups hexokinase hydrocarbon chains hydrocarbon-coated agaroses hydrophobic interaction immobilized ligand ion exchange chromatography ionic strength isozyme kinase kinetic lactate dehydrogenase lectin ligand ligand concentration litres M.J. Harvey matrix method mg/ml molecular molecule Mosbach NaCl NAD+ NADH non-biospecific non-specific nucleotide oxamate P.D.G. Dean packed peak Pharmacia phosphate buffer plasma polyacrylamide porous precipitate preparation procedure protein purification residues salt sample Sephadex Sepharose Sepharose-4B serum Shaltiel sodium solution sorbent spacer arm specific Spherosil substrate technique tion Ultrogel washed