Clinical Applications of PCR
Y. M. Dennis Lo
Humana Press, Mar 2, 1998 - Science - 353 pages
The polymerase chain reaction (PCR) is one of the most important molecular biological methods ever devised, with numerous applications to cli- cal molecular medicine. Since its description in 1985, PCR has undergone tremendous improvements, and many variations on the basic PCR theme have been published. With such a large volume of PCR-related literature, a clinical scientist wishing to use the technique will have a difficult task loc- ing the relevant information to implement it effectively. There is thus clearly a need for an up-to-date volume with detailed protocols to facilitate the setting up of those techniques most relevant to clinical applications. Unlike some other books on this topic, Clinical Applications of PCR includes only methods that are of direct relevance in clinical settings. The book is organized in three parts: an introductory section, a section on general methodology, and a final section with specific clinical applications. The first section covers the basic principles of PCR and is most useful to those new to molecular diagnosis. The next chapter includes useful tips for setting up a PCR laboratory. Section 2 then outlines some of the most commonly used PCR-based techniques in molecular diagnosis. Section 3 includes carefully chosen examples that represent typical applications of PCR in diverse clinical fields, encompassing hematology, oncology, genetics, and microbiology.
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Introduction to the Polymerase Chain Reaction
Setting Up a PCR Laboratory
Amplification from Archival Materials
23 other sections not shown
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A-RFLP agarose gel aliquot alleles analysis annealing Applications of PCR ARMS primer assay biotinylated cDNA chromosome Clinical Applications concentration contamination cycles denaturation detection digestion diluted DNA fragments DNA polymerase DNA sequences dNTPs EDTA Eppendorf ethanol ethidium bromide Exon fetal cells fluorescent gene genetic genomic genomic DNA human hybridization Incubate IS-PCR labeled lane markers maternal blood mg/mL MgCl2 microsatellite mitochondrial molecules mRNA mtDNA Note Nucleic Acids Nucleic Acids Res nucleotide oligonucleotide PCR amplification PCR buffer PCR product PCR reaction pellet peripheral blood pipet plate point mutations polymerase chain reaction polymorphism prenatal diagnosis probes proteinase protocol quantitative reagents restriction enzyme reverse transcription RNA extraction room temperature RT-PCR samples sensitivity single cell slides sodium solution specific SSCP staining standard step sterile Store strand Subheading Taq polymerase target sequence technique template thermal thermal cycler tissue Tris-HCl tube tumor cells volume Wash