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A Guided Tour of the Minefield
High Copy Number Plasmids Compatible with Commonly Used Cloning Vectors
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activity adenovirus admid affinity amino acids antibody assay bacterial binding Biochem Biol BioTechniques buffer C-terminal cDNA cell lines cleavage codon construct copy number culture detection Eaton Publishing Edited by Quinn efficient encoding epitope epitope tag Escherichia coli eukaryotic expression system expression vectors Figure FLAG FLAG-tagged fragment Function Analysis Edited fused fusion protein GCN4 gene expression Gene Function Analysis genome green fluorescent protein human incubated induced inserted intein IPTG lanes leucine zipper levels ligation linkers Lu and Michael luciferase lysates mammalian cells method molecular mRNA multiple cloning multiple cloning site mutation oligonucleotides P-gal pCMV-Tag PCR products peptide plasmid primers promoter protein expression purification Quinn Lu reaction receptor recombinant protein replication reporter gene RNA polymerase signal splicing strain Stratagene subcloning substrate subunit synthesis target tein terminus tetracycline tion transcription transfection transformed transgenic translation Vectors for Gene viral virus vitro vivo Western blot yeast