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Structure of a DNAunwinding Protein and
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3H]thymidine Acad acid activity alkaline analysis assay ATPase bacteriophage BamHl binding Biochemistry Biol Biology cells chromosome circular cistron-A protein cleavage cleotide cloning Cold Spring Harbor ColEl complex containing copy number covalently Dept digested DNA chains DNA fragments DNA gyrase DNA helicase DNA molecules DNA polymerase DNA replication DNA sequence DNA synthesis dnaB dnaG dUTP EcoRI EcoRl electrophoresis enzyme Escherichia coli Figure fraction function gene gene-A protein gene-V genetic Helinski Hindlll Hpall hybrid incubated indicated initiation isolated Kornberg labeled lambda Molecular mtDNA mutants nascent DNA Natl novobiocin nucleotide sequence nucleotides Okazaki oligonucleotide oriC origin of replication oxolinic acid phage plasmid DNA primase Proc protein pulse-labeled purified radioactivity reaction rep protein replication fork replication origin replicons restriction endonuclease RFI DNA sedimentation shown single-stranded SS DNA strain strand structure subunit sucrose gradient supercoiling template tion transcription unwinding uracil viral vitro vivo