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Design of the study scientific rationale and chemical selection
survey of the literature E Waters Swansea
Evaluation of the mutagenic activity of 4dimethylaminoazobenzene
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4-cyanodimethylaniline 4-dimethylaminoazobenzene absence of S9 acetyl CoA Alderley Park aneuploidy animals ARO/RAT S9 Aroclor Aroclor-induced rat liver Aroclor-induced S9 aromatic amines Ashby bacterial mutagenicity benzidine benzidine BZD bone marrow Cancer Carcinogenesis carcinogens CDA Sample chicken S9 Chinese hamster chromosome aberrations cultures cytogenetic DAB and CDA detection DMSO DNA repair dose levels Drosophila effect enzyme Evaluation experiments Figure Forward mutation gene conversion genetic genotoxic glucose hepatocytes incubated induced laboratories mammalian cells medium metabolic activation metabolites mg/kg mice micronuclei micronucleus test microsomes mouse S9 mutagenesis mutagenic mutagenic activity mutagenicity test mutation frequency Mutation Res NT NT observed obtained Parry plate positive control preincubation presence of S9 protocol rat liver S9 S9 concentration S9 fraction S9 mix S9 preparations Saccharomyces cerevisiae Salmonella typhimurium Short-term Tests strain TABLE test chemicals test compound tissue toxicity treatment ug/ml ug/plate uninduced rat urine vitro vivo volume W79 cells yeast