Complement Methods and Protocols

Front Cover
B. Paul Morgan
Springer Science & Business Media, 2000 - Science - 279 pages
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Although the complement system-a vital part of the body's defense against bacteria-was discovered more than a century ago, its study has undergone a renaissance with the identification of its regulatory molecules and the realization that these molecules can be used therapeutically. In Complement Methods and Protocols, B. Paul Morgan and a team of expert laboratorians present a comprehensive set of readily reproducible methods to study this critical system. These cutting-edge techniques are suitable both for the basic scientist interested in understanding complement's mechanisms of activation and for the clinical scientist wishing to quantify its activation, and range from the purification of its components to generating complement-deficient mice by gene deletion. Additional techniques presented include procedures for the analysis of complement function, for the study of its regulators, for detection of its activation in vivo, and for the identification of its autoantibodies. Comprehensive and cutting-edge, Complement Methods and Protocols offers today's basic and clinical investigators powerful tools for the analysis of the role of complement in human pathophysiology and disease, as well as its therapeutic regulation.
 

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Contents

The Complement System An Overview
1
Purification of Complement Components Regulators and Receptors by Classical Methods
15
Immunoaffinity Methods for Purification of Complement Components and Regulators
53
Measurement of Complement Hemolytic Activity Generation of ComplementDepleted Sera and Production of Hemolytic Intermediates
61
Measurement of Complement Lysis of Nucleated Cells
73
Functional Assays for Complement Regulators
83
Immunochemical Measurement of Complement Components and Activation Products
103
Complement Deposition in Tissues
113
C1Inhibitor Antigenic and Functional Analysis
159
Autoantibodies to Complement Components
173
Allotyping of Complement Components
193
Complement and Immune Complexes
203
Knocking Out Complement Genes
215
Inherited Complement Deficiencies in Animals
229
Appendices
249
Sources of C Components and AntiC Antibodies
258

Complement Regulators and Receptors in Tissues
123
Measurement of C3 Fragment Deposition on Cells
131
Screening for Complement Deficiency
139
cDNA Accession Numbers for C Components Regulators and Receptors
260
Index
263
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