Computer Operation for Microscope Photometry

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CRC Press, Nov 25, 1997 - Science - 256 pages
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Suitable for both microscopists seeking computer skills and PC enthusiasts interested in light microscopy, this interdisciplinary text explores the capabilities of the computer-assisted light microscope. Written in clear, simple language, the book explains how computer technology expands the usefulness of the light microscope in spectrophotometry, fluorometry, polarimetry, spatial scanning, and related fields.
Beginning with the basic features of light microscopy and personal computer interfacing, the text explains how to make photometric measurements and covers spectrophotometry, stepper motors, and server motors. Polarized light and video image analysis complete this introduction to the field. While software examples are provided to illustrate specific techniques, most operations are described as generalized algorithms that can be adapted to any appropriate high-level language, and used with almost any configuration of the microscope. The book suggests new experiments to inspire further study. Promising new areas of interest, such as the use of fluorescence and polarization, are also included.
Computers have radically changed the field of light microscopy in recent decades. Computer Operations for Microscope Photometry helps you master the new techniques.
 

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Contents

Introduction
1
Illuminators
29
Shutters and Apertures
35
Photometry
59
Monochromators and Spectrophotometry
85
Mechanical Stages and Scanning
101
Polarized Light
113
Fluorescence
137
Video
153
Photodiode Array Spectrography
165
Liberating the Microscope with Fiberoptics
175
Programming the Sample Environment
203
References
219
Index
233
Copyright

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