Confocal Raman Microscopy

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Thomas Dieing, Olaf Hollricher, Jan Toporski
Springer Science & Business Media, Jan 14, 2011 - Technology & Engineering - 292 pages
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Confocal Raman Microscopy is a relatively new technique that allows chemical imaging without specific sample preparation. By integrating a sensitive Raman spectrometer within a state-of-the-art microscope, Raman microscopy with a spatial resolution down to 200nm laterally and 500nm vertically can be achieved using visible light excitation. Recent developments in detector and computer technology as well as optimized instrument design have reduced integration times of Raman spectra by orders of magnitude, so that complete images consisting of tens of thousands of Raman spectra can be acquired in seconds or minutes rather than hours, which used to be standard just one decade ago. The purpose of this book is to provide the reader a comprehensive overview of the rapidly developing field of Confocal Raman Microscopy and its applications.
 

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Contents

1 HighResolution Optical and Confocal Microscopy
1
2 Introduction to the Fundamentals of Raman Spectroscopy
21
3 Raman Instrumentation for Confocal Raman Microscopy
43
4 Software Requirements and Data Analysis in Confocal Raman Microscopy
61
5 Nanospectroscopy of Individual Carbon Nanotubes and Isolated Graphene Sheets
91
6 Raman Spectroscopy and Confocal Raman Imaging in Mineralogy and Petrography
111
7 Raman Microspectral Imaging of Cells and Intracellular Drug Delivery Using Nanocarrier Systems
137
8 Confocal Raman Microscopy in Pharmaceutical Development
165
9 Characterization of Therapeutic Coatings on Medical Devices
203
10 Raman Imaging of Plant Cell Walls
225
11 Confocal Raman Imaging of Polymeric Materials
237
12 Stress Analysis by Means of Raman Microscopy
259
Index
279
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