Cryobanking the Genetic Resource: Wildlife Conservation for the Future?
Paul Watson, William V. Holt
CRC Press, Feb 16, 2001 - Nature - 480 pages
There is a growing awareness of a worldwide reduction in biodiversity and the urgent need to develop ways to redress the problem. This is the first major book devoted to the subject of genetic resource banking (GRB) and its role in preserving global animal biodiversity.
In Cryobanking the Genetic Resource, expert contributors provide the non-specialist with an overview of the subject and the practical techniques associated with GRB. The book presents a basic introduction to the concepts, and then points the way to relevant literature for those who wish to develop practical applications. The first section deals with the potential contribution of GRB to biodiversity protection, while the second section offers an introduction to the basic cryobiology of gametes and embryos.
In the first section, genetic considerations for planning interventions are discussed, together with disease control measures. This section explores related reproductive technologies necessary for the use of cryopreserved gametes and embryos, as well as a range of other issues such as ownership and security of stored material, and the size and location of cryopreserved material. The second section offers a thorough review of the literature on cryopreservation of spermatozoa, oocytes, and embryos. It gathers species into related groups for ease of reference, and does not assume extensive specialist knowledge so that newcomers to the field can make the best use of the available research.
This book is an essential resource for zoologists and conservationists with an interest in genetic management and captive breeding, and also for students involved in biodiversity issues, conservation, and assisted reproduction.
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of Genetic Resource Banking 85
PART B Survey of the Literature on Cryopreservation
Miscellaneous Mammals 391
acrosomal integrity ambient temperature animals artificial insemination assessment Biol blastocysts boar bovine breeding programmes captive cells concentration conservation cooling rates cryo Cryobiology cryopreservation cryoprotectant culture diluent dilution disease DMSO domestic cat donors effects egg yolk ejaculate electroejaculation embryo cryopreservation embryo transfer etal female follicles follicular freezing and thawing fresh frozen semen frozen-thawed gametes genetic diversity genetic resource banking germplasm glycerol gonadotrophins Holt hormone immature oocytes Johnston Kasai koala Leibo liquid nitrogen Loskutoff Maisse males marsupial medium method Mohor gazelle morulae mouse embryos mouse oocytes non-domestic normal oestrus oocytes osmotic ovarian ovaries ovulation pellets permeability plasma membrane populations post-thaw motility Poultry Sci pregnancy preservation procedure PrOH protocols rainbow trout recovery Reprod Rodger samples semen collection slow cooling solution species sperm cryopreservation sperm motility spermatozoa storage stored studies successful sucrose survival techniques Theriogenology tissue Tselutin ungulates viability vitrification vitro fertilization Watson Whittingham WILDT Wishart zona