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15 pound pressure 24 hours 30 minutes acetic acid Adjust reaction agar agglutination Agglutination test alcohol 95 Animal inoculation antigen aqueous autoclave autoclave at 15 bacilli Bacteria blood agar4 Board of Health boil broth buffer solution c.c. Add c.c. serum carriers cells cent citrated colonies color culture darkfield diphtheria Dissolve distilled water drop dysentery Eberthella typhosa Enteric Diseases examine exudate Feces filter Flexner glass slide grams guinea pig high dry hours at 37 incubate at 37 infection infectious mononucleosis laboratory Lactose MACROGAMETOCYTE medium METHOD microscope slide Motility Negative nutrient agar oil immersion parasites paratyphoid Peptone phenol phosphate plates Positive potassium reagent refrigerator Report rise in titer room temperature saline Salmonella Salmonella enteritidis Salmonella paratyphi sediment serological Shiga slant smear Sodium chloride specimens spinal fluid sputum stain by Gram's Sterile swabs Sterile test tubes surface suspension tion titer transurent uncolued tularemia ture typhoid uncolued & transurent urine