Cytochemical staining methods for electron microscopy
The series Practical Methods in Electron Microscopy, edited by Audrey M. Glauert has an international reputation as a unique source of practical information for all electron microscopists. Each book of the series starts from first principles, assuming no specialist knowledge, and is complete in itself. The series will eventually cover the whole range of techniques for electron microscopy. In this latest volume in the popular series, Peter Lewis and David Knight describe in practical detail the whole range of staining techniques for electron microscopy, including contrast staining for ultrastructural studies, specific cytological staining methods, and enzyme cytochemical techniques. The chemical basis of cytochemical methods is discussed and analysed in a straightforward manner with great clarity. In consequence this book will help the beginner to choose between the sometimes bewildering variety of published technical procedures. Electron microscopists will find this volume to be an invaluable and compact source-book for all those cytochemical techniques that have proved to be both reliable and convenient to use. As with previous titles in this popular series, the unique type of practical guidance provided will be of value for many years to come.
What people are saying - Write a review
We haven't found any reviews in the usual places.
Introduction by PR Lewis and D P Knight
Cytological staining methods by D P Knight
General cytochemical methods by D P Knight
5 other sections not shown
aldehyde aldehyde fixative aldehyde groups alkaline phosphatase aqueous ATPase biochemical buffer at pH cacodylate calcium capturing agent carbohydrates Cell Biol cerium cerous chemical chloride cholinesterase citrate components concentration containing contrast cytochemical cytochemistry cytochrome dehydrogenase demonstration diffusion distilled water electron microscope electron microscope level embed enumerated procedure enzyme enzyme activity epoxy resin esterases ethanol ferricyanide Fix tissue fixation formaldehyde Glauert glutaraldehyde glycogen grids histochemical techniques histochemistry hydrogen hydrolysis incubation medium indoxyl inhibition inhibitor Karnovsky lead citrate lead ions lectins light microscope level lipids localization Magnification membrane mitochondria nucleic acids obtained osmium tetroxide oxidase oxidation peroxidase potassium pre-incubation precipitate prepared produce proteins proteoglycans reaction product reagents resin sections Rinse room temperature selective staining silver silver proteinate slices or sections sodium specific staining methods staining procedure staining solution stock solution structures substrate succinic suitable sulphate sulphide tetrazolium salts tissue slices tracer Tris/maleate ultrastructural ultrathin sections uranyl acetate