DNA Fingerprinting in Plants: Principles, Methods, and Applications, Second Edition

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CRC Press, Feb 28, 2005 - Science - 472 pages
3 Reviews
Given the explosive development of new molecular marker techniques over the last decade, newcomers and experts alike in the field of DNA fingerprinting will find an easy-to-follow guide to the multitude of techniques available in DNA Fingerprinting in Plants: Principles, Methods, and Applications, Second Edition. Along with step-by-step annotated protocols, the authors fully discuss the technical aspects and modifications of existing techniques, the influence of reaction components and conditions, and the analysis of the results.

This second edition has been completely revised to address the exponential changes in the field since the first edition, focusing on PCR-based techniques but also including more sophisticated ones. The authors include numerous case studies to illustrate applications of the methods, more than 1600 references to the literature, and descriptions of reagent formulation, equipment, and computer programs used for evaluating molecular marker data. They compare the various methods, including the costs and benefits of each, helping readers determine which is best suited to a particular application.

The well-rounded, cross-referenced, and unified nature of this book makes it intrinsically easier to follow than the edited, multi-authored books currently available. It is an absolute necessity on the lab bench of anyone involved in plant research, DNA profiling, and molecular markers.

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An Important Source of Variation in Eukaryotic Genomes
Chapter 2 Detecting DNA Variation by Molecular Markers
Chapter 3 Laboratory Equipment
Chapter 4 Methodology
Chapter 5 Evaluation of Molecular Marker Data
Chapter 6 Applications of DNA Fingerprinting in Plant Sciences
Chapter 7 Linkage Analysis and Genetic Maps
A Comparison
Appendix 1 Plant DNA Isolation Protocols
Appendix 2 Commercial Companies
Appendix 3 Computer Programs Dealing with the Evaluation of DNA Sequence Variation and Molecular Marker Data
Appendix 4 Web Pages of Interest
Back cover

SNiPs and Chips for DNA and RNA Profiling

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Page 362 - Feinberg AP and Vogelstein B. (1983). A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity.
Page 417 - D. (1987) A sequence in M13 phage detects hypervariable minisatellites in human and animal DNA. Science 235, 683-684.
Page 415 - Triglia, T., Peterson, MG, and Kemp, DJ (1988) A procedure for in vitro amplification of DNA segments that lie outside the boundaries of known sequences. Nucleic Acids Res. 16, 8186.
Page 414 - TC 1994. Comparison of RFLP and RAPD markers to estimating genetic relationships within and among cruciferous species. Theoretical and Applied Genetics, 88: 973-980.

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