DNA Viruses: A Practical Approach

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Alan Cann
New York, 1999 - Medical - 314 pages
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DNA Viruses: A Practical Approach groups together the major experimental methods currently employed to study DNA viruses, from the fundamentals of virus culture to novel techniques such as surface plasmon resonance spectrometry and realtime PCR analysis of drug resistance mutations in clinicalisolates. Chapter 1 provides an overview of the extraction, purification and characterizations of virus DNA, but also covers the fundamentals of DNA virus culture. Chapters 2 and 3 describe approaches to the molecular investigation and mutagenesis of DNA virus genomes. Chapter 4 considers DNAvirus replication and then chapters 5 and 6 describe methods to study transcription control. Chapters 7 to 9 consider aspects of the pathogenesis of DNA virus infections. The final chapter describes the current technology being applied to the development of DNA virus vectors for gene delivery. Thisvolume will therefore be of interest to all those working on DNA viruses whether in academia, industry or clinical research.

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Contents

Investigation of DNA virus genome structure
15
Sequence assembly
31
Sequence analysis
39
Copyright

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About the author (1999)

Alan J. Cann is at University of Leicester.

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