DNA Viruses: A Practical Approach
New York, 1999 - Medical - 314 pages
DNA Viruses: A Practical Approach groups together the major experimental methods currently employed to study DNA viruses, from the fundamentals of virus culture to novel techniques such as surface plasmon resonance spectrometry and realtime PCR analysis of drug resistance mutations in clinicalisolates. Chapter 1 provides an overview of the extraction, purification and characterizations of virus DNA, but also covers the fundamentals of DNA virus culture. Chapters 2 and 3 describe approaches to the molecular investigation and mutagenesis of DNA virus genomes. Chapter 4 considers DNAvirus replication and then chapters 5 and 6 describe methods to study transcription control. Chapters 7 to 9 consider aspects of the pathogenesis of DNA virus infections. The final chapter describes the current technology being applied to the development of DNA virus vectors for gene delivery. Thisvolume will therefore be of interest to all those working on DNA viruses whether in academia, industry or clinical research.
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Investigation of DNA virus genome structure
27 other sections not shown
acetate aciclovir acid activity adenovirus agarose gel aliquots analysis antibody assay binding blot buffer cell lines cell type cellular centrifugation chromatography cloned column complex concentration containing contigs cytokine denatured described in Protocol digestion dilution dishes DMEM DNA polymerase DNA replication DNA virus DNA viruses EDTA electrophoresis elute Equipment and reagents ethanol extract fractions fragments gene expression genome genomic DNA glycerol growth medium hybridization infection interaction isolation lysate membrane Method mg ml MgCl2 monolayer mutagenesis mutagenic mutations NaCl nucleotide oligonucleotide overlay pellet penciclovir plaque plasmid plates prepared primers probe promoter protein of interest Protocol 11 purification reaction recombinant Remove reporter gene restriction enzyme Resuspend room temperature sample SDS-PAGE sequence serum solution sterile supernatant template tissue culture transcription factors transfection transfer Tris-HCl pH tube vector viral cytokine-binding protein viral genome Virol virus stock vitro volume Wash wild-type