Diagnosis of Protozoa and Worms Parasitic in Man

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Williams & Wilkins, 1921 - Helminths - 72 pages
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Page 66 - ... connective tissue at the base of the mesentery around the pancreas, behind the pericardium, and behind the abdominal aorta and suprarenal capsules; microfilariae (Plate VI, Fig.
Page 21 - The smear is prepared for microscopic examination by rubbing out a minute bit of the feces by rolling it on a round applicator stick in a small drop of normal salt solution and then in an adjacent drop of iodin-eosin stain. A single cover is placed on both drops and the smear is ready for immediate examination.
Page 21 - ... stain. A single cover is placed on both drops and the smear is ready for immediate examination. Living flagellates and unstained cysts appear in the unstained part. In the stained area the bacteria, faecal particles and the intestinal yeasts (except the larger forms) stain at once.
Page 17 - Place the end of another slide near the drop of blood at an angle of about 30 degrees with the shorter end of the slide. Draw this slide along until it touches the drop. When the blood has spread along the edge, push the slide fairly rapidly toward the other end. A thin even film will result covering about one half of the slide.
Page 43 - Fig. 82. (1.) where c is a co-efficient to be found empirically. As it is difficult, if not impossible, in the present state of our knowledge, to distinguish between that factor in the power of a...
Page 22 - ... iron-hsemotoxylin method is necessary. This in brief is as follows: Spread the intestinal or rectal contents in a thin layer over about one half the area o? a 3x1 glass slide. Before this has a chance to dry drop it face downward into a dish containing Schaudinn's fixing solution. This is made up of a saturated solution of mercuric chloride in distilled water, 200 cc ; 95 per cent, alcohol, 100 cc; and glacial acetic acid, 15 cc Leave in this solution for about ten minutes. The slide should then...
Page 41 - ... of men in the Army from the Southern States during the war. In this method a fecal sample is thoroughly mixed with concentrated brine. The coarse float is forced below the surface with a disc of No. 0 steel wool and the container allowed to stand for about an hour for the eggs to ascend. The surface film is then looped off onto a slide and examined without a cover glass. The microscope must be focused on the surface of the drop on the slide. This method gives fine concentration and a very clear...
Page 70 - ... same species. They are common parasites of the cat and civet cat and probably only incidental in man. 2. Sub-family Bunostominae. Small buccal capsule, with .aperture narrowed anteriorly by plates with cutting edges springing from the sides, and more or less covering the ventral half of the aperture; coils of genital tubes very numerous and close. Externo-dorsal ray thin more especially at the root; spicules of the male barbed at ends. Necator americanus (Plate VIII, Figs. 1 and 3). Head bent,...
Page 71 - The male measures 15 to 25 cm. in length and about 3 mm. in thickness; the female is 20 to 40 cm.
Page 21 - ... parasites found. The best preparations result from the use of Schaudinn's alcoholic-sublimate iron-haemotoxylin method. The methods of fecal diagnosis employed depend somewhat on the accuracy of the results desired and the ability to obtain and use special apparatus. The Donaldson iodin-eosin-smear method seems to be the quickest and easiest. Concentration methods give a slightly higher percentage of positives and the Schaudinn iron-haemotoxylin smear method just mentioned is very useful in checking...

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