Drosophila: a practical approach
David B. Roberts
IRL Press at Oxford University Press, 1998 - Medical - 389 pages
Drosophila--the scientist's favorite laboratory organism--is a key subject for studying genetics, development, and behavior. The second edition of this popular hands-on guide brings the methodology up-to-date, with contributions from the leading researchers in the field. Chapters include the elements of Drosophila biology and genetics; mutagenesis; transposons--gene tagging and mutagenesis; chromosome mechanics: the genetic manipulation of aneuploid stocks; enhancer traps; looking at embryos; immunolabelling of Drosophila; population and ecological genetics; behavior, learning, and memory; cell culture; and preparation of nucleic acids.
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acid adult agar alleles allow aneuploid antibody assay autosomal autosynaptic stocks behavioural Biol bottles breakpoints buffer carry cells Centrifuge clones collection coverslip cross culture deletions dissected dorsal Drosophila effector gene eggs element embryos enhancer trap enzyme Equipment and reagents ethanol excision extraction Figure flanking flies FlyBase formaldehyde frequency GAL4 genetic genomic genomic DNA genotype germ band grid tube heptane heterozygotes heterozygous homozygotes homozygous imaginal discs Incubate insert inversion labelling laboratory larvae lines males mapping marker medium melanogaster Method microcentrifuge tubes mutagen odour block pellet Petri dish phenotype pipette plasmid plasmid rescue plate population preparations procedure produce progeny protein Protocol recessive lethal mutations recombination recovered region remove room temperature score screen selection sequence slide solution specific stage sterile strain sucrose supernatant tion tissue Transfer translocation transposase transposon vector vials virgin females Wash wild-type yeast