Effects of Heterologous Ribosomal Binding Sites on the Expression of the Lacz Gene of Escherichia ColiA.C. Looman, 1986 - 107 pages |
Contents
INTRODUCTION | 1 |
CHAPTER 2 GENERAL MATERIALS AND METHODS | 20 |
CHAPTER 3 THE EXPRESSION VECTOR pKL203 AND ITS USE IN THE STUDY | 28 |
7 other sections not shown
Common terms and phrases
16S rRNA 2nd codon Acad Acids Res amino acids AUG codon AUG initiation codon B-gal B-galactosidase Bacillus BamHI basepairing Berkhout Biol Boer Chapter clones codon choice codon usage coli constructs deletion digested EcoRI EDTA efficiency enzyme expression vector Fiers fusion protein gene expression Gly Asp HaeIII heterologous highly expressed genes immunoprecipitation incubation initiation codon interaction IPTG isolated Kastelein Knippenberg lac mRNA lacZ gene ligated to pKL203 Looman lysate lysis MacConkey messenger mRNA mutations Natl niet Nt sequences Nucl nucleotides phage plasmid plasmid containing PMS2 Proc prokaryotes protein production protein RBS RBSs region replicase gene replicase RBS ribosomal binding sites SD nts SD sequence second codon secondary structure Smal start codon synthetic TABLE tion transcription transformants triplet Tris pH 7.4 tRNA tRNA species tufA tufB Val Val vector pKL203 vitro werd wild-type