Electroanalytical Methods Of Biological Materials

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Anna Brajter-toth, James Chambers
CRC Press, Mar 21, 2002 - Science - 552 pages
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This text details contemporary electroanalytical strategies of biomolecules and electrical phenomena in biological systems. It presents significant developments in sequence-specific DNA detection for more efficient and cost-effective medical diagnosis of genetic and infectious diseases and microbial and viral pathogens. The authors discuss the latest advances in amperometric biosensing, capillary electrophoresis, DNA amplification and detection, single-cell neurochemistry, in vivo electrochemistry, and electrochemical immunoassay. They also present recent techniques to evaluate the reaction properties of complex electron-transport enzymes and describe the use of fast-scan cyclic voltammetry (FSCV) to study the mechanisms and kinetics of dopamine neurotransmission, among other topics.
 

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Contents

1 Charge Migration Through the DNA Double Helix
1
2 Electrochemical DNA Biosensors
25
Amplified and Specific Electronic Transduction of DNA Sensing Processes in Monolayer and ThinFilm Assemblies
39
4 Direct Electrochemistry of Proteins and Enzymes at Electrodes
107
5 Voltammetric Investigations of IronSulfur ur Clusters in Proteins
137
6 Polyion and Surfactant Films on Electrodes for Protein Electrochemistry
190
7 Electrochemistry of Peroxidases
228
8 Mechanisms and Kinetics of Neurotransmission Measured in Brain Slices with Cyclic Voltammetry
247
10 Milestones of Electrochemical Immunoassay at Cincinnati
324
11 Electrochemical Detection of Peptides
362
12 Microfabrication of Electrode Surfaces for Biosensors
392
13 Electrocatalytic Determination of Biochemical Compounds
409
14 Electrodes Based on the Electrical Wiring of Enzymes
430
15 Capillary ElectrophoresisElectrochemistry
451
Ultrahigh Sensitivity Analysis of Amino Acids and Peptides by Capillary Liquid Chromatography with Electrochemical Detection
480
Index
515

9 Electrochemical Monitoring of Exocytosis from Individual PC12 Cells in Culture
270

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