Electron Microscopy: Methods and Protocols[

Front Cover
M. A. Nasser Hajibagheri
Springer Science & Business Media, 1999 - Medical - 283 pages
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In Electron Microscopy Methods and Protocols, well-practiced experts describe in detail the key electron microscopy techniques used for examining cells, tissue, biological macromolecules, molecular structure, and their interactions. With emphasis on cryotechniques for quantitative biological X-ray microanalysis, the book also includes those methods that use antibodies to locate proteins within cells and that prepare and analyze nucleic acids, proteins, and protein-nucleic acid complexes. Numerous immunogold labeling techniques for precise ultrastructural localization, distribution, and quantitation of macromolecules in cryo-fixed or chemically-fixed cells are described in sufficient detail to provide practical insight into their advantages and limitations. Electron Microscopy Methods and Protocols offers both newcomers and established researchers wanting to expand their repertoire of cutting-edge electron microscopy techniques-each optimized for reproducibility and robust results-today's gold-standard laboratory manual.
 

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Contents

General Preparation of Material and Staining of Sections
1
Negative Staining of Thinly Spread Biological Particulates
13
Preparation of ThinFilm FrozenHydrated Vitrified Biological Specimens for Cryoelectron Microscopy
31
The Production of Cryosections Through Fixed and Cryoprotected Biological Material and Their Use in Immunocytochemistry
49
HighPressure Freezing for Preservation of High Resolution Fine Structure and Antigenicity for Immunolabeling
77
The Application of LR Gold Resin for Immunogold Labeling
99
LowTemperature Embedding in Acrylic Resins
111
Quantitative Aspects of Immunogold Labeling in Embedded and Nonembedded Sections
125
Electron Microscopic Enzyme Cytochemistry
159
In Situ Molecular Hybridization Techniques for Ultrathin Sections
167
Preparation of the Fission Yeast Schizosaccharomyces pombe for Ultrastructural and Immunocytochemical Study
183
Preparation of DoubleSingleStranded DNA and RNA Molecules for Electron Microscopy
209
Applications of Electron Microscopy for Studying ProteinDNA Complexes
229
XRay Microanalysis Techniques
245
Index
277
Copyright

Microwave Processing Techniques for Electron Microscopy A FourHour Protocol
145

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