Electron Microscopy: Principles and Techniques for BiologistsNew edition of an introductory reference that covers all of the important aspects of electron microscopy from a biological perspective, including theory of scanning and transmission; specimen preparation; darkroom, digital imaging, and image analysis; laboratory safety; interpretation of images; and an atlas of ultrastructure. Generously illustrated with bandw line drawings and photographs. Annotation copyrighted by Book News, Inc., Portland, OR. |
Contents
Specimen Preparation for Transmission Electron Microscopy | 16 |
DEVELOPMENT OF THE ELECTRON FIXATION | 19 |
Freezing Method for Specimen | 27 |
WASHING | 34 |
EMBEDDING CELL FRACTIONS | 41 |
Specimen Preparation for Scanning Electron Microscopy | 48 |
SPECIMEN DRYING TECHNIQUES | 54 |
REPLICATION PROCEDURES | 61 |
Chapter 13 | 323 |
Spherical Objects | 330 |
ASSUMPTIONS AND CONDITIONS | 336 |
COMPUTERASSISTED STEREOLOGY | 337 |
Freeze Fracture Replication | 342 |
HOW TO PRODUCE A REPLICA | 347 |
COMPLEMENTARY REPLICAS | 354 |
Chapter 15 | 358 |
SPECIMEN STORAGE | 69 |
Ultramicrotomy | 72 |
Ultramicrotomy | 74 |
TYPES OF ULTRAMICROTOME | 82 |
GRIDS | 90 |
THE ULTRAMICROTOME | 97 |
CRYOULTRAMICROTOMY | 109 |
REFERENCES | 117 |
NEGATIVE STAINING | 130 |
REFERENCES | 146 |
Chapter 6 | 173 |
CONTRAST AND THREEDIMENSIONALITY | 203 |
PREPARING THE TRANSMISSION | 205 |
Specimen Staining and Contrast Methods for Transmission Electron Microscopy 120 | 217 |
The Transmission Electron Microscope 148 | 220 |
STEREO IMAGING WITH THE SEM | 221 |
SPECIALIZED INSTRUMENTATION | 231 |
DIGITAL SCANNING ELECTRON | 238 |
DARKROOM PRINTING | 250 |
REFERENCES | 261 |
THE ANTIGENANTIBODY | 263 |
GENERAL CONSIDERATIONS | 274 |
Chapter 10 | 282 |
REFERENCES | 290 |
AutoradiographyRadioautography | 292 |
EMULSION USED | 293 |
IN SITU HYBRIDIZATION USING | 305 |
Miscellaneous Localization and Enhancement Techniques | 310 |
Miscellaneous Localization | 311 |
REFERENCES | 365 |
Analytical Electron Microscopy | 368 |
MICROSCOPES USED FOR DETECTING | 371 |
INFORMATION OBTAINABLE USING | 378 |
Intermediate and High Voltage Microscopy | 396 |
ADVANTAGES OF HIGH VOLTAGE Sharpening 429 | 397 |
Tracers | 408 |
Image Processing and Image Analysis by Computer | 414 |
CONTRIBUTIONS OF HIGH VOLTAGE LookUp Tables Thresholding Binary | 432 |
Area | 438 |
Interpretation of Micrographs | 442 |
REFERENCES | 475 |
THE CYTOSKELETON | 506 |
The Nuclear Envelope and Nuclear | 514 |
THE LYSOSOMAL SYSTEM | 556 |
PEROXISOMES OR MICROBODIES | 563 |
EXTRACELLULAR MATERIAL | 571 |
SPECIAL FEATURES OF PLANT | 579 |
BACTERIA | 585 |
VIRUSES | 592 |
| 608 | |
Chapter 20 | 616 |
CHEMICAL SAFETY | 622 |
FIRE SAFETY | 628 |
HOTLINES AND OTHER RESOURCES | 639 |
Appendix A Review Questions and Problems | 641 |
| 653 | |
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Common terms and phrases
acid angle antibody antigen aperture astigmatism autoradiography backscattered electrons biological specimens block face buffer carbon Chapter chemicals coated condenser lens containing contrast Courtesy crystal Cytochem dehydration density detector determine diffraction diffusion pump elec electron beam electron micrograph electron microscopy embedding emission emulsion enzyme epoxy ethanol evaporated exposure filament film fixation fixative focus freeze fracture frozen glass glass knife glutaraldehyde Golgi grid high voltage holder knives labeled lenses light microscope magnification membrane metal method micro Microsc microtome microtubules mitochondria molecules negative staining objective lens obtained organelles osmium tetroxide particles photographic placed plastic possible prepared procedure propylene oxide protein remove replica resin rinsed scanning electron scanning electron microscopy secondary electrons slide solution speci specimen block spot stereology structure surface technique temperature thickness thin sections tion tissue transmission electron microscope tron types ultramicrotome ultrastructural ultrathin uranyl acetate usually vacuum viewing screen volume