Electron Microscopy of Plant Cells

Front Cover
C Hawes
Academic Press, Dec 2, 2012 - Science - 476 pages
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Electron Microscopy of Plant Cells serves as manual or reference of major modern techniques used to prepare plant material for transmission and scanning electron microscopy. There have been other books that generally discuss electron microscope methodology. This book focuses on problem areas encountered through the presence of tough cell walls and large central vacuole. It details preparative techniques for botanical specimens.
Each of the nine chapters of this book covers the basic principles, useful applications, and reliable procedures used on the method of electron microscopy. Other topics discussed in each chapter include the general preparation and straining of thin sections, quantitative morphological analysis, and enzyme cytochemistry. This book also explains the immunogold labelling, rapid-freezing methods, and ambient- and low-temperature scanning electron microscopy among others.
This book will be invaluable to general scientists, biologists, botanists, and students specializing in plant anatomy.
 

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Contents

Chapter 1 General Preparation and Staining of Thin Sections
1
Chapter 2 Stereoelectron microscopy
67
Chapter 3 Quantitative Morphological Analysis
85
Chapter 4 Enzyme Cytochemistry
105
Chapter 5 Immunogold Labelling
181
Molecular Cytology Goes Ultrastructural
219
Chapter 7 RapidFreezing Methods and their Applications
257
Chapter 8 Ambient and LowTemperature Scanning Electron Microscopy
313
Chapter 9 Microanalysis
415
Index
457
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