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Fixation Washing and Dehydration
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acetone acid added aldehyde allow antibody Appendix aqueous Araldite autoradiography biological block carbon film cells cent solution Chapter chemical coated components containing convenient dehydration described developed diluted distilled water edge electron microscope embedding medium emulsion enzyme Epon epoxy resin ethanol evaporated exposure ferritin fixation floating formaldehyde Formvar fracture freeze frozen sections g Water glass glass knives glutaraldehyde glutarate grains grids infiltration knife knives lanthanum layer lead stains light microscope liquid material membranes methacrylate method micrographs microtome ml Water molecules monolayer monomer mounting negative staining object obtained osmium fixative osmium tetroxide oxide particles particular peroxidase phosphotungstic acid photographic piece of tissue plastic polymer polymerization possible post-fix preparation problem procedure produce protein radioactive reaction recommended replica resolution salts shadowing slide sodium solvent specimen stabilized step structures substrate suitable technique thick thin sections tion uranyl acetate usually vacuum washing water bath water surface